The Study Of The Relationship Of Male Infertility And Free Radical

Infertility is an international medical and physical difficult problem. Infertility affects up to 10-15% of the sexually active population, and in 40-50% of cases, a male factor is involved, either as a primary problem or in combination with a problem in the female partner. Therefore, the study and treatment of male infertility is of great importance in scientific and medical research. In recent years, the generation of reactive oxygen species (ROS) in the male reproductive tract has become a real concern because of their potential toxic effects at high levels on sperm quality and function. In order to have an extensive research on these potential toxic effects, a series of researches had been executed. 4437 semen samples for these experiments were selected from fertile and infertile persons who came to First, Second and Third Clinical Hospitals of Jilin Universitity to have a semen test since 2004 July to 2006 February. The semen test included: measurment of sperm motility, sperm morphology, sperm survival rate, DNA integrity, semen total antioxidative capacity, levle of semen malonaldehyde, level of reactive oxygen species (ROS) producted by sperm and seminal leukocyte, test of percentage of sperm survival, death and apoptosis. The results indicated that a high level of reactiv oxygen species would cause a sigenificant descend in sperm motive velocity and sperm rate parameters, an ascend in sperm motive manner parameters, and a decline in sperm DNA integrity (p<0.05). It would also result a decline in sperm survival rate, and a raise in sperm death and apoptosis rates (p<0.05). A high level of seminal reactive oxygen species may be a major reason for male infertility, especially the quantities of reactive oxygen species from sperm and leukocyte and that result in the leukocytal provocation. The quantities of reactive oxygen species of semen and that result in the leukocytal provocation were higer in infertile group than that in fertile group. The quantity of malonaldehyde was higer in infertile group than that in fertile group (p<0.05). Howere, no significantly change of quantity of reactive oxygen spesies was seen between before and after the freeze of sperm, and the antioxide material has no improved effect. These results demonstrated that seminal reactive oxygen species will lead to a all-round decline in sperm quality, including sperm motility, sperm DNA integrity, survival rate. Seminal reactive oxygen species may lead a tremendous influence on male fecundity, andthe level of seminal malonaldehyde can be a quota for asthenospermia and leukocytospermia. In a word, seminal reactive oxygen species play a greatly influence on male infertility, it can be an important factor for the drop of sperm quality, and an important factor for male infertility.