Study On The Relationship Between Lipopolysaccharide And Semen Quality Of Male Reproductive Tract Infection

In recent years, with the increase of social development, the deterioration of the environment, living habits and the society pressure, male infertility is increasingly concerned. According to the World Health Organization(WHO) regulations, Married couples not to take any contraceptive measures, a year later cannot be pregnant because of male factors, known as the male infertility. Male infertility is a global disease that seriously affect male reproductive health, social and family stability. There are many factors that cause male infertility, including congenital abnormal development of reproductive organs, genetic diseases, endocrine diseases, reproductive system infection, inflammation and physicochemical factors etc.. The reproductive tract infection is an important cause of male infertility, accounted for 15% of male infertility patients. So the study of male reproductive tract infection plays an important role in male infertility.Gram negative bacteria is the common bacteria in the male reproductive tract infection and lipopolysaccharide(LPS) is an important component of gram-negative bacterial cell wall, and meanwhile is the main pathogenic factor. LPS can cause damage to sperm through a variety of ways. Toll like receptor(TLR) is a cell membrane receptors for LPS the main a highly conserved, containing pathogen pattern recognition receptor transmembrane transporter protein receptor, which may be pathogens(including LPS) activation and the rapid start of the inflammatory response. The male reproductive tract infection may cause the increase in the number of leukocytes in semen and white cell surface expression of TLRs, LPS through toll like receptors(TLRs) activated white blood cells causing cytokine gene expression was enhanced and on sperm damage. Studies have confirmed the presence of TLRs on human spermatozoa, LPS can be directly applied to the surface of sperm TLRs damage to sperm, thus affecting male fertility.The research of the male urogenital tract infection on sperm damage and to explore in urogenital tract infection in semen of the existence of LPS, analysis the effect of LPS on semen quality, and further study of the damage effects of LPS on sperm. This study collected semen samples from 113 patients with genital tract infection, detection of LPS concentration in semen. By means of quantitative detection of LPS in the semen, understand the clinical seminal LPS in patients with genital tract infection situation and the concentration range. Analysis of LPS infection in patients with semen concentration, leukocyte concentration and semen parameters, to investigate the effects of infection on male infertility. Inspection found that the reproductive tract infection patients, 26 cases of semen in LPS was detected positive semen in LPS concentration range for(13.8 ±12.68) EU ·ml-1. The reproductive tract infection patients and healthy adult male sperm quality decreased significantly compared. No significant difference have been detected in urogenital tract infection in LPS positive group and LPS negative group between semen quality. In order to further study of LPS on sperm damage effects, In this study, the high sperm motility resuspended solution and 0.1ug·ml-1LPS were incubated, through the detection of sperm motility, sperm reactive oxygen species, sperm mitochondrial membrane potential and apoptosis rate to observe the damage effect of LPS on sperm. Test results found that reactive oxygen species(ROS) in sperm to 559±42.7, compared with the control group increased significantly; sperm mitochondrial membrane potential was 11.1±2.61, compared with the control group decreased significantly, but sperm motility and sperm apoptosis rate did not change significantly.In summary, the existence of LPS in semen of male genital tract infection, can significantly affect the semen quality of male genital tract infection, but in the LPS positive group and LPS negative group had no statistical difference. LPS and high sperm motility resuspended solution were incubated with education has a direct effect on sperm, can cause sperm increased reactive oxygen species, sperm mitochondrial membrane potential decreased, thus affecting sperm quality, but did not affect the sperm apoptosis and activity.