Expression And Clinical Significance Of Syndecan-1, E-Cadherin And MMP-9 In Human Sporadic Colorectal Carcinoma

Objectives: The study was designed to discuss the roles of Syndecan-1, E-Cadherin and MMP-9 protein in the carcinogenesis and development of human sporadic colorectal carcinoma (SCC) and the likely molecule mechanism by investigating the expression of Syndecan-1, E-Cadherin and MMP-9 protein in SCC.Methods: Immunohistochemistry SP method was used to determine the expression level of Syndecan-1, E-cadherin and MMP-9 protein in surgically resected specimens of 52 SCC and 18 control normal colorectal mucosa.Results: 1.The positive expression rates of Syndecan-1 protein was significantly lower in SCC (65.38%) than in normal colorectal mucosa (100%,p<0.05). No correlation were found between expression level of Syndecan-1 protein and patient sex, age and tumor size (p>0.05). The positive rate of Syndecan-1 protein was lower in poor differentiation tissues than in well differentiation tissues (p<0.01). The deeper extent of invasiveness, the lower Syndecan-1 protein positive rate was (p<0.01). The Syndecan-1 protein positive rate was lower in SCC with lymph node metastasis than in those without lymph node metastasis (p<0.01). Expression level of Syndecan-1 protein decreased in Dukes C, D stage of SCC compared with Dukes A, B stages of SCC (p<0.01). 2. The positive expression rates of E-Cadherin protein was significantly lower in SCC (61.54%) than in normal colorectal mucosa (100%, p<0.01). Significant correlation existed between the decreased expression of E-Cadherin and the depth of tumor invasion, the lymph-node metastasis, Dukes stages (p<0.05). 3. The positive expression rates of MMP-9 protein was significantly higher in SCC ( 86.54%) than in normal colorectal mucosa (11.11 %,p<0.01). Significant correlation existed between the decreased expression of MMP-9 and the grade of differentiation, the depth of tumor invasion, the lymph-node metastasis, Dukes stages (p<0.05). 4. The expression of Syndecan-1 and E-Cadherin in SCC existed significantcorrelation (rJ=0.333,/?<0.05). And a significant negative relationship was observed between the expression of Syndecan-1 and MMP-9 in SCC (rJ=-0.570,^<0.05).Conclusions: The data suggest that the expression level of Syndecan-1, E-cadherin and MMP-9 protein was closely related to biological behavior of SCC. The decrease or deletion of Syndecan-1, E-cadherin protein expression and the over-activation of MMP-9 protein may result in invasiveness and metastasis. Determination of Syndecan-1, E-Cadherin and MMP-9 is valuable for diagnosing SCC, and evaluating malignancy extent and prognosis.