The Effect On Expression Of Invasion Relative Proteins And Migration Ability In Human Hepatocellular Carcinoma Cell Transfected With Exogenous P16

Objective:To investigate the effect on expression of invasion relative proteins and migration ability in human hepatocellular carcinoma cell transfected with Exogenous p16.Methods:4The effect on migration ability of Human Hepatocellular Carcinoma cell transfected with exogenous p16 was assayed by in vitro scratch wound healing assay and transwell chamber assay. Protein level of vascular endothelial growth factor (VEGF) was detected by immunocytochemical staining. Protein levels of nm23 MMP-9 and TIMP-1 was assessed by Western blot.Results:(1) The result of in vitro scratch wound healing assay showed that the migration abilities of SMMC-7721-p16 cells is obviously decreased compared with that of SMMC-7721-VECTOR and SMMC-7721 cells.(2) The migrated cells was decreased and reached to 39.50±2.72 per vision. A significant difference was tested between the experiment group and control groups (P<0.05).(3) The expression of vascular endothelial growth factor (VEGF) was decreased in SMMC-7721 cells which transfected with pl6 gene.(4) The expressions of nm23 and TIMP-1 were increased while of MMP-9 was decreased in SMMC-7721 cells transfected with p16 gene.Conclusion:...