The Expression Of Survivin Messenger RNA And CD133 Messenger RNA In Acute Leukemia And Its Clinical Significance

Objective To evaluate the expression of survivin mRNA and CD133 messenger RNA in acute leukemia (AL) patients and its clinical significance .Method Bone marrow samples from 41 patients with acute leukemia were examined and bone marrow samples from 10 healthy persons were used as control. Reverse transcriptase polymerase chain reaction (RT-PCR) was performed to detect survivin mRNA and CD133 mRNA expression in specimens. The results were analyzed relatively.Result (1) The positive rate of survivin mRNA in the acute leukemia (78.0%) is higher than that in normal control (1/10) ( P < 0.005). There were no relationship between survivin mRNA expression and age, sex, white blood cell count, type of AL, alkaline phosphatge , lymph node enlargement, liveromegaly or splenomegaly (P >0.05). There was relationship between survivin mRNA expression and response to chemothraphy (P < 0.05). (2) The positive rate of CD133 mRNA in the acute leukemia (42.0%) is higher than that in normal control (0/10) (P < 0.005). There were no relationship between CD133 mRNA expression and age, sex, white blood cell count, alkaline phosphatge, lymph node enlargement, liveromegaly or splenomegaly and response to chemothraphy ( P>0.05). There was relationship between CD133 mRNA expression and type of AL (P < 0.05), M₄ was the highest but M3 was the lowest. (3) The expression of survivin mRNA in AL was significantly correlated with the expression of CD133 mRNA (r_s=0.6429). (4) In...