Experimental Investigation On Effect Of Cell Immunity After Naloxone Hydrochloride Treated Traumatic Brain Injury In Rats

Objective: We expected to explore the effects ofβ-EP,CD₄⁺,CD₈⁺ and IL-2 in cell immunity by testing the levels of these cytokines in plasma of rats suffering from TBI. We plan to demonstrate the effects and potential mechanisms of cell immunity on rats with TBI by observing the changes ofβ-EP,CD4+,CD8+ and IL-2 in serum after the treatment of naloxone.Methods: (1)The models of severe brain injury were produced by gas percussion. (2) The levels of CD4+ and CD8+ in serum of rats were detected by Flow cytometer(FCM) ;The levels ofβ-EP in serum of rats were detected by radioimmunoassay(RIA); The levels of IL-2 in serum of rats were detected by Enzyme-linked immunoabsorbent Assay (ELISA).Results:1. CD₄⁺ and IL-2 significantly decreased at 6h in serum following TBI(P<0.01;P<0.05), and their valley values existed respectively at 1d,3d after TBI(P<0.01).Whileβ-Ep and CD₈⁺ increased at 6h(P<0.01;...