Experimental Investigation On Inflammatory Respone After Naloxone Hydrochloride Therapy To Traumatic Brain Injuru In Tats

Objective: To discuss the effects of NF-κB and TNF-αin second brain injury, by detecting their levels in brain tissue of rats suffering from TBI. To investigate the effects and probable mechanisms of naloxone hyrochloride on rats with TBI, by observing the changes of NF-κB and TNF-αin brain tissue after the treatment of different dosage naloxone .Methods: (1)The model of severe brain injury in rats were produced by gas percussion. (2)The histopathological changes of brain tissue in rats were observed by HE stain. (3) The expression of NF-κB in brain tissue of rats were detected by immunohistochemical method ;The levels of TNF-αin brain tissue of rats were detected by enzyme-linked immunoabsorbent assay (ELISA).Results:1. NF-κB and TNF-αsignificantly increased at 6h in brain tissue of rats following TBI(P<0.05), and their peak value was respectively at 1d,3d after TBI(P<0.01). They keep on high level to 7d(P<0.05 or P<0.01). NF-κB and TNF-αwere linear positive correlation (r=0.657,p<0.01).2. NF-κB and TNF-αin brain tissue of rats suffering from TBI decreased after treatment with naloxone. Compared to the curative effect of the small dosage naloxone and the large dosage naloxone, it is significant difference(P<0.05 or P<0.01).Conclusions:1. NF-κB and TNF-αin brain tissue increased in early period after TBI, and keep on high level to 7d, and caused inflammatory response and the processes of SBI.2. Naloxone showed protective effects by regulating the levels of NF-κB and TNF-αand relieved the second injury caused by the inflammatory cytokines in rat brain tissue after TBI.3. Naloxone treatment of TBI should follow early,large,adequate–term therapy.4. The potential therapeutic mechanisms of Naloxone on the inflammatory response after TBI: Inhibitaing of NF-κB activation and TNF-αlevels, and blocking inflammation cascade reaction;Inhibiting activation of inflammatory cells and the synthesis and release of inflammatory cytokines;Inhibting inflammatory cell inflitration gathered,reducing secondary hypoxic-ischemic inflammatory response ,etc.