Clinical Study On The Effect Of Erythrocyte Immunity Function After Naloxone Hydrochloride Treated Traumatic Brain Injury In Children

Objective: To explore the changes and mechanism of erythrocyte immunity function(EIF)in children after traumatic brain injury(TBI); to study the effects of Naloxoen(NAL) on erythrocyte immunity function of children after traumatic brain injury and further to investigate its probably mechanism.Methods:(1)To choose children after TBI as test group and divided them into mild group and moderate to severe group according to the extent of injury meanwhile to choose the common sick children at the same period as control group.(2)The test group was divided into Naloxoen-treated group and conventional therapy group .The former group was treated with different doses Naloxoen instead of normal sodium in the later group.(3)the content of serumβ-endophin(β-EP) in children after TBI was tested by radioimmunoassay(RIA).(4)Red Blood Cell-C3b Receptor Rate( RBC- C3bRR) and Red Blood Cell- Immune Complex Rate( RBC-ICR) of children after TBI were measured by methed of yeast fungus. Results:(1)Compared with the control group, RBC-C3bRR of children after TBI decreased significantly while RBC-ICR increased significantly(P<0.01).Moreover, the moderate to severe group had more manifest difference than the mild group(P<0.05).(2)Within 24h after injury, RBC-C3bRR began to decrease in both mild group and moderate to severe group. However, in the former it would be declined to normal level at 14 days after injury whereas in the later it would decrease more significantly and would not decline.(3)The increasing of RBC-ICR in mild group was not significant within 24h after injury . But at 72h RBC-ICR began to increase significantly and reach the peak at 7d,then it began to drop, but still was higher than control group. The increasing of RBC-ICR in moderate to severe group was more significant and had not dropped at 14d after injury yet.(4)The serumβ-EP level in both mild severe group was significantly increased in 24 hours after TBI, and moderate to severe group was significantly higher. After declining, they were still higher than control group at 14d after injury.β-endorphin was negatively correlated with RBC-C3bRR (r=-0.865,P<0.05)and positively with RBC-ICR (r=0.644,P<0.05) in mild group;and negatively correlated with RBC-C3bRR (r=-0.635,P<0.05)and positively with RBC-ICR (r=0.746,P<0.05)in severd group.(5) Compared with the conventional therapy group, serumβ-EP in naloxone-treated group was significantly decreased (P <0.05). RBC-C3bRR increased and RBC-ICR decreased (P <0.01). Erythrocyte immune dysfunction had noticeably improved, but there was still differences compared with the control group.results:(1)EIF in children after traumatic brain injury decreased significantly,which showed that RBC-C3bRR decreased and RBC-ICR increased.(2)Decreasing of EIF in moderate to severe group was more markedly than in mild group which indicated erythrocyte immune dysfunction would be more severe accompanied with more severe injury.(3) Serumβ-EP in children After traumatic brain injury increased significantly, and there was a linear correlation with RBC-C3bRR,RBC-IC, which demonstrated erythrocyte immune dysfunction was related with increasing of theβ-EP.(4)Treated with naloxone, the content ofβ-EP in children after TBI decreased meanwhile erythrocyte immune dysfunction was alleviated. There was a linear correlation. Naloxone was confirmed to regulate the erythrocyte immune dysfunction through competitive inhibition ofβ-EP in children after TBI.