Studies On Chemical Constituents Of Antitumour From Rhamnus Crenatus And Schisandra Chinensis

This dissertation reports the studies on the chemical constituents and activity of Rhamnus. crenata and Schisandra chinensis. The plant of Rhamnus. crenata (Rhamnaceae) distributed broadly in the world has been used in the treatment of arthritis, scabies and tineas in China as a folk medicine.Fifteen coumpounds were isolated from the aerial parts of R. crenata. Based on the spectra analysis their structures were identified as crenatoside (1), crenatol (2), torachrysone B (3), torachrysone (4), 2-Acetyl-1,8-naphthalenediol (5), physcion (6), chrysophanol (7), emodin (8), emodin-1-glucopyranoside (9), physciondianthrone (10), 2-methoxystypandrone (11), aromadendrin (12), β-sitosterol (13), 1-triacontanol (14) and 1-dotriacontanoic acid (15). Among them compound 1 is a new chromone glucoside derivative, 2 is a new indane derivative and 3 is a new naphthalin derivative and the others were isolated from R. crenata for the first time.Schisandra chinensis belonging to genus Schisandra is a commonly used traditional Chinese drug. Eight dibenzocyclooctadiene lignans, wuweizisu C (16), wuweizisu B (17), schisandrol B (18), schisandrol A (19), schisanhenol (20), gomisin M₁ (21), gomisin M₂ (22) and gomisin R (23) were isolated from the seed oil of S. chinensis and their structures were characterized unambiguously on the basis of spectroscopic evidences.The cytotoxicity and antitumour activities of the crude extracts and compounds were evaluated. The crude extracts of R. crenata and S. chinensis were found to have potent cytotoxicity and antitumour activities in vitro. Among components 1-15, only compound 11 exhibited moderate antitumour activity and the others were found to be inactive.