IGF-1R Antibody Inhibiting Formation Of Hypertrophic Scars And Influencing The Expression Of Bcl-2 And Bax In Rabbit Ears

Objective To investigate the effect of insulin-like growth factor-1 receptor antibody (IGF-1R Ab) on hypertrophic scars and the expression of Bcl-2 and Bax in the rabbit ears.Methods Establish the animal models of hypertrophic scars on rabbit ears. The rabbits were divided randomly into 4 groups as follows, ①solvent control[saline solution (0.09%Nacl), A group]. ②IGF-1R Ab 30ng/20ul/d [B group]. ③IGF-1R Ab 60ng/20ul/d[C group]. ④IGF-1R Ab 120ng/20ul/d[D group]. And there are 4 rabbits as normal control and blank control on average. After epithelization, A group were injected into scars by mini-injector on a fixed time everyday, B～D group with different drug concentration ,all for 7 days. All specimens were used by the pathology tectology(HE and VG) and immunohistochemistry staining technique(SP) to observe the general, hypertrophic index(HI), fibroblast count, collagen area density on area and the expression of Bcl-2 and Bax .Results In comparison with A group, day 3 after administration, the HI of C group had obviously decreased(P<0.05) and D group had highly obvious contrast(P<0.01). Fibroblast count and AA of C and D group had also been less than A at the same day(P<0.01). Day 7,11,20 and 30 after disposal, 3 indexes took on greatly apparent distinction between C or D and A(P<0.01). It was significant difference in HI or fibroblast count on day 3 between C and B group(P<0.05),but more in AA (P<0.01), and indexes of D group had been lower than B (P<0.01). And the decreased degree of 3 indexes in C and D group became less than that in B group from day 7 to day...