Study On Immune Tolerance On Orthotpic Liver Transplantation In The Inbred Rat Infected With Echinococcosus Multicularis

Objectives: To investigate the immune characteristics of rats infected with Echinococcosus multicularis(E.m) and its effects on the graft by using BN-to-LEW orthotopic liver transplantation(OLT) models. Methods: Two-cuff method was adopted in the BN-to-LEW OLT models. 96 health BN and 96 LEW rats were used in the current study, the LEW rats consited of 48 health rats and 48 rats with E.m infection.The animals were randomly divided into five groups as follows: Group A normal controls were produced by (OLT in the same strain)from LEW-to- LEW; Group B.strain controls were produced by (OLT in the different strain)form BN-to-health LEW; Group C: E.m stimulated growp were produced by BN-to-LEW rat with E.m infection for 3 months; Group D: drug treated group were produced by BN-to-health LEW and Tacrolimus (FK506) after OLT(0.1mg/(kg·d)); Group E: E.m cambined drug treated group were produced by BN-to-LEW rat with Em infection for 3 months and Tacrolimus(FK506) after OLT(0.1mg/(kg·d)). The OLT procedure was considered as succesed model when the LEW rat can be survived over 48h. If any modle was died within 48h ,the rat was excluded in the study. The LEW ser??, liver graft, spleen samples were collected in 1,3,5,7 days after OLT respectively. Eight LEW rats in each group were remained for observation of their the survival days. Results: The mean survival times for each group were 54.13±16.62d for group A, 4.75±1.58d...