The Effect Of Marrow-Supplementing And Blood-Engendering Granule On Colony-Forming Unit-Fibroblast Of Wistar Rats With Aplastic Anemia And Toxicologically Examine

Objective: To investigate the long term toxicity of Marrow-Supplementing and Blood-Engendering Granule for normal Wistar rats and the effects of Marrow-Supplementing and Blood-Engendering Granule on bone marrow and peripheral blood of Wistar rats with aplastic anemia.Method: (1) The biochemistry of normal Wistar rats were observed and the key organs were cut for calculating the viscera coefficient and pathological examination after being treated with Marrow-Supplementing and Blood-Engendering Granule at higher, middle and low dosages for 180 days. (2)After shined on the ⁶⁰Co-Y ray with 8.0Gy, Wistar rats caught aplastic anemia and were treated with Marrow-Supplementing and Blood-Engendering Granule at higher, middle and low dosages for three weeks, The colony formation of CFU-F of Wistar rats in aplastic anemia were observed by semi-solid culture of bone marrow.Results: (1)After given medicine for 180 days, the groups treated with Marrow-Supplementing and Blood-Engendering Granule had no significant difference in biochemistry data, and only very few light changes could recover without the medicine in 15 days;the viscera coefficient of all groups had no significant difference;the low and middle dosage had no significant influence to major viscera and the higher dosage led to light changes of blood clot, bleeding and edema within heart, kidney and liver, but the changes were deteriorated. (2)The low, middle and higher dosage medicine could make the productivity of CFU-F cultured outside the body. It was a significant difference to compare with the model group and the group with the zaizhangshengxue tablet (P<0.05);it could be seen hematopoietic tissue was multiplied,well-distributeand recovery when the tissue slices of pathology were observed.Conclusion: Marrow-Supplementing and Blood-Engendering Granule had no side-effect or significant influence to biochemistry and viscera coefficient. It could promote the CFU-F forming, stimulate HSC multiplying and recover the hematopoietic tissue, so it-could change the state of bone marrow inhibition of Wistar rats caught aplastic anemia and improve their living quality. Marrow-Supplementing and Blood-Engendering Granule showed more effective than zizhangshengxue tablet.