Acanthopanax Senticosus Injecyion On Cerebral Ischemia-Reperfusion Injury

Objective:To investigate the protective effect of acanthopanax senticosus injection (ASI) on cerebral ischemia-reperfusion injury in rats about bcl-2, NF-kB and p53,and to explore the therapeutic and mechanism of acanthopanax senticosus injection (ASI) on cerebral ischemia-reperfusion injury.Methods:Two hundred rats were devided into model group, sham operated group ,therapy group and control group randomly.Every group were injected liquor natrii chloridi isotonicus , acanthopanax senticosus injection and Ligustrazine Hydrochloride respectively by intraperitoneal injection for ten days.Then the cerebral ischemia-reperfusion injury model was estabalished . Each group was divided into seven parts at 2,4,8,12,24,48 and 72h according to the time of cerebral ischemia-reperfusion ,Every group remained four rats at each time point after removing dead and unformed model ones .All rats wre executed ,taken out brain and fixed at special time. We detected the effect of the expression of bcl-2 . NF- kB and p53 in the cerebral ischemia-reperfusion injury four groups applying to immunohisto chemistry method .Results:The study showed that the expression of NF-kB and p53 was lower in ASI group and Ligustrazine Hydrochloride positive control group than in model group,and the expression of bcl-2 was higher(P<0.05);the expression of bcl-2 and p53 didn't discover and only a little NF- kB appeared which was lower in the sham operated group than in model group and medicine group.Conclusion:can increase the expression of bcl-2 and decrease the cell apoptosis at cerebral ischemia tissue.(2)ASI can cut down the expression of p53 which can lead to nervous cell apoptosis.(3)ASI can lower expression of NF- kB and decrease cerebral ischemia-reperfusion injury.