The Relationship Between Helicobacter Pylori And Primary Intrahepatic Stones

Objective: To study the relationship between helicobacter pylori (H. pylori) infection and primary intrahepatic stones.Methods: Regular anaerobic and Hp cultures were performed for all 49 samples ( include 39 samples of primary intrahepatic stones and 10 samples of the controls ). Helicobacter pylori DNA in all bile samples and mucous of intrahepatic samples and the controls were detected by PCR, and Western-blotting was performed to detect H. pylori associated protein in all bile samples.Results: The regular, anaerobic and Hp cultures were found 18 positive of regular and anaerobic in 39 bile samples of primary intrahepatic stones and 1 positive of regular in the controls. All the samples Hp cultures were found no bacteria growing. PCR showed positive in 26 (66.67 %) of 39 bile samples, 11(52.38 %) of 21 mucous samples, 9 showed positive both in mucous and bile samples,of which 17(65.38%) contained anti -CagA antibodies ,14(53.85%) contained VacA. UreB and UreA were detected in 19 (73.08%) and 12 (46.15%) samples respectively. There were also Vacuolating cytotoxin associated 30Kd (12,46.15%) glycoprotein found in bile samples of patients with intrahepatic stones. While only 1 positive in the controls of H. pylori DNA and no associated protein of H. pylori infection in the negative of H .pylori DNA. Conclusions: It is suggest that presence of Hp-DNA in intrahepatic bile and mucous , and associated protein of H. pylori infection in most bile samples may be represent risk factors for the formation of intrahepatic stones.