Clinical Value Of Antibody Typing And Helicobacter Pylori Antibiotics Susceptibility Testing For Diagnosis And Theapy Of Helicobacter Pylori

Helicobacter pylori(H.pylori)infection has been widely recognized as a major cause of chronic gastritis,peptic ulcer and gastric cancer,and hasbeen classified as a Class I carcinogen by the World Health Organization.The infection rate of H.pylori is increasing year by year in China.H.pylori is not only related to various digestive diseases,but also related to various parenteral diseases.Therefore,we should have the correct understanding of H.pylori infection and accept the active treatment,in order to reduce the rate of being attacked by H.pylori related disease.The aim of this study was to investigate the value of antibody typing and breath testing in diagnosis of H.pylori infection and explore the significance of drug sensitivity testing for H.pylori infection,and guide clinical work.Part ? Clinical value of antibody typing for diagnosis of helicobacter pylori infectionObjectiveTo investigate the rate of Helicobacter pylori(H.pylori)infection by using antibody typing detection,analyze the value of H.pylori antibody type in diagnose of H.pylori infection and its risk factors.MethodsThe patients accepted H.pylori antibody typing from July 2017 to Dec 2017 were selected from Jinan Central Hospital affiliated to Shandong University.According to H.pylori antibody type,all patients were divided into 3 groups,type I,type II and negative,whether or not with H.pylori related diseases were recorded.UBT(Urea breath test UBT)were performed one week before or after H.pylori antibody type detection.ResultsIn total,there were 462 patients accepted H.pylori antibody type detection,the totalinfection rate was 66.2%,43.7% were defined type I,the most common antibody type were cagA,vacA,ureA,ureB positive,type II infection rate was 22.5%.Age,H.pylori related diseases,appendicitis and drinking were statistically different among the three groups(P<0.05),which were independent risk factors for the H.pylori infection.The patients with type I were more commom than type II(P<0.05).The positive coincidence rate of UBT and antibody typing was 80.55%,and the negative coincidence rate was 86.88%,the total coincidence rate was 84.53%.ConclusionThe H.pylori infection rate in this area was high.Older,someone with H.pylori related diseases,appendicitis,drinking were the high risk factors of the H.ylori infection.UBT combine with antibody type could benefit in diagnosing and classifying H.pylori Infection.Part ? Clinical value of helicobacter pylori isolated?verification and antibiotics susceptibility testingObjectiveTo analyse the resistance rate of the H.pylori to 14 antibiotics by using the microbiological detection.MethodsThe patients accepted the ure breath test and the results were positive from December 2018 to January 2019 were selected from Jinan Central Hospital.At the same time,gastroscopy were performed and biopsy from mucosa of antrum of the patients with H.pylori infection were used for developing the pylori and antibiotics susceptibility testing.ResultsIn total,there were 34 accepted antibiotics susceptibility testing.The results showed H.pylori was resistant,and the resistance rates of amoxicillin?amoxicillin clavuric acid?cefixime ? gentamicin ? azithromycin ? clarithromycin ? levofloxacin ? sparfloxacin ?metronidazole?tinidazole?rifampicin to H.pylori were 5.9%?2.9%?11.8%?5.9%?11.8%?32.4%?41.1%?5.9%?82.4%?55.9%?2.9%.Respectively,H.pylori were absolutely sensitiveto the tetracycline ? doxycycline ? furazolidone.However,5.9 percent of the pylori were absolutely sensitive to the 14 antibiotics,while the resistance rate of H.pyloti to one class of antibiotc?two classes of antibiotics?three or more than three classes of antibiotics were44.1%?38.2%?11.8%.The resistance rate of levofloxacin?metronidazole in the patients over40 years old was higher than those younger than 40;the resistance rate of two classes of antibiotcs in male were less than that in female.ConclusionThere were a range of antibiotics insensitive to H.pylori,the resistance rate of H.pylori to one classes of antibiotc?two classes of antibiotics also were high.Susceptibility testing could analyse the sensitivity of H.pylori to the antibiotics,be helpful for choosing the sensitive antibiotics,and improve the eradication rate of H.pylori in clinic.