Relationship Between Gene Polymorphism Concerned 5-HT And Functional Bowel Disorders, And Evaluation Of Psychological Factors With Functional Bowel Disorders

Objective:1.To investigate the relationship between serotonin transporter(SERT or 5-HTT) gene-linked polymorphism (5-HTTLPR), 17bp varied number tandem repeats(VNTRs) polymorphism within intron 2 and A218C polymorphism within intron 7 of tryptophan hydroxylase-1(TPH-1)gene and irritable bowel syndrome (IBS), functional diarrhea(FDi) and functional constipation(FC).2.To evaluate the psychological factors on patients with functional bowel disorders(FBDs).Materials and methods:1.Patients and controlThe study group comprised 51 patients with D-IBS,58 patients with C-IBS , 38 patients with A-IBS ,32 patients with FDi,41 patients with FC who fulfilled the Romeâ…¡criteria for FBDs and 48 healthy subjects.2.SamplesBlood samples were taken from leukocytes in serum, DNA was extracted from whole blood with standard techniques and kit for DNA extracted .3.GenetypingUse PCR and PCR-RFLP to compare 5-HTTLPR and VNTRs polymorphism of serotonin transporter gene and A218C polymorphism of tryptophan hydroxylase -1 gene in FBDs patients.