The Correlate Study Of Children's VEGF And PIGF In Astrocytoma Grading Prognosis

Background and objective: Astrocytoma are common intracranial neoplasms exhibiting morphologic or immunophenotypic evidence of an origin from epithelium cells of nerve,accounting for 60% of all primary intracranial tumours.[1] Astrocytoma is the most common intracranial neoplasms of children, accounting for 55% of all children's primary intracranial tumours. They are a heterogeneous group of diseases with respect to complicated histopathological classification, overelaborate grading and abviously disparity in immunophenotypic features,their prognoses are quite different and difficult to evaluate. We know little about the pathogenesis of astrocytoma as yet. Recent researches show that blood vessels supply the nutrition of tumour cell growth,and astrocytoma are rich in vessels, so it is especially important to study vascular growth factor family for evaluation of astrocytoma prognosis. PIGF is a new member of vascular endothelial growth factor (VEGF) family which has been detected that it has different expression in several tumours. However, the study about astrocytoma is extremely little.To make clear the influencing factor of children's astrocytoma prognosis, guide clinical treatment and elevate patient survival rate, we retrospectively studied 105 cases of astrocytoma by immunohistochemistry according to the new classification of WHO in 2000.The results are statistical analysis.Materials and Methods1. Materials: 105 cases of astrocytoma are from General Hospital of Shenyang Military Region (1981-2005). The age of patients including 63 males and 62 females is from 0.5 to 14 years old. The sections are recut for 4pm sections and stained with routine HE.2. Methods:Immunohistochemistry: All of these 105 cases of astrocytoma arestained with immunohistochemical regents. It includes CD34,VEGF,PIGF. They are used Streptavidin/Peroxidase(SP) method. VEGF and P1GF are located both in the plasma of the tumour cells.Evolution3.1 Evolution of the MVD: The microvessel was labeled by CD34. The stained capillary vessel and microvessel were accounted according to the standard of the Weidner. Any individual brown endothelial cell and cell cluster were account as one while the area of the hemocoele higher than 8 times of diameter of the red cells should be ignored. The methods of count: Count the CD34 positive microvessels under 200×. Four different views were selected random for counting the microvessels. The mean value is the MVD ( number/x200)3.2 Evaluation of VEGF and PIGF:Positive VEGF expression defined as brown particles existed in cytoplasm and membrane.3.3 Statistical analysis:All statistical analyses were performed using the Statistical Package for SPSS ll.5. The association of variables was evaluated using the x2 test, t-test and Spearman analysis.Results:Immunohistochemistry:All of these 105 cases,the levels of expression with PIGF in three grading children's astrocytoma are different. Besides the positive correlation between VEGF and MVD was analysed in highly malignancy astrocytoma, as well as PIGF and MVD, and we also detected an absolute positive correlation with VEGF and P1GF.Conclusions:1.The level of expression of PIGF has significant difference in four grading children's astrocytoma.2.The level of expression of VEGF has significant difference in four grading children's astrocytoma.3.Both VEGF and PIGF produce a marked correlation in children's astrocytoma angiogenesis, especially in highly malignancy astrocytoma with MVD. It provides physicians with a new method to increase the survival rate by using anti- angiogenesis remedy treating children's astrocytoma.