The Expression And Clinical Significance Of LRP16, AR, Cyclin E Gene In Hepatocellular Carcinoma

OBJECTIVE:1. Identify the expressive condition of LRP16,AR,Cyclin E in hepatocellular carcinoma.2. Analyze the relation between the protein expression level of LRP16, AR, Cyclin E and clinically pathologic factor of hepatocellular carcinoma respectively.3. Investigate the correlation among LRP16, AR, Cyclin E in hepatocellular carcinoma.METHODS:Using Western-blot technique to detect the protein expression level of LRP16, AR, Cyclin E in hepatocellular carcinoma tissues, paired peritumor tissues and normal hepatocellular tissues from 42 patients with hepatocellular carcinoma. (All patients' HBsAg is positive. No patients received any preoperative anticancer therapy. All patients have complete clinical data.). Analyze the relation between the protein expression level of LRP16, AR, Cyclin E and clinically pathologic factors of hepatocellular carcinoma. Immunohistochemical staining of AR in hepatocellular carcinoma tissues were observed.RESULTS:According to the results of Western blot, the carcinoma tissues' protein expression level of LRP16 is lower than paired peritumor and normal tissues. The negative expression of LRP16 in HCC is relevant with tumor size, vascular encroachment and well differentiation; The carcinoma tissues' protein expression level of AR is higher than paired peritumor and normal tissues. The over-high protein expression level of AR in HCC is relevant with vascular encroachment and well differentiation, AR protein was present in the nuclei of all hepatocellular carcinoma cells. The carcinoma tissues' protein expression level of Cyclin E is higher than peritumor and normal tissues. The over-high protein expression levelof Cyclin E in HCC is relevant with tumor size, vascular encroachment and poordifferentiation.CONCLUSION:1.Androgen receptor does not regulate LRP16 directly, maybe it regulate LRP16 indirectly through estrogen receptor.2.LRP16 is one of the regulatory factors of Cyclin E which produce a marked effect during the process of tumorigenesis. LRP16 play a different roles in different carcinomas.3.AR can up-regulate Cyclin E expression, promote cell division, so AR can influence the hormone-dependent tumor cell's proliferation.