Studies On The Active Constituents And Quality Control Of Cynanchum Artatum Bunge

Cynanchum atratum Bunge, locally called'paiwei'(Asclepiadaceae), as one species of traditional Chinese medicine, widely distributs all around China especially in North China, Yunnan, Guizhou provinces. Cynanchum atratum Bunge has been used as a medicine for the treatment of hectic fevers ,acute urinary infection and abscesses for a long time in China. Morden research on pharmacology have indicated that the extracts of Cynanchum atratum have the activities of anti-inflammatory, analgesic activity, anti-asthma et al.In order to study further the resource of"Paiwei", to illustrate the mechanism of the anti-inflammatory and analgesic activities and to find more active compounds, we use the bioactivity-screening on anti-inflammatory and analgesic activities to determinate the active fractions, and then to make the emphases isolation of this fraction. We are conducting a thorough solvent extraction, using non-polar macroreticular resin AB-8,HP-20,sephadex LH-20,ODS,HPLC,MPLC,silica gel chromatography and semi-preparative columns, we isolated 32 compounds from the chloroform fraction and 24 structures of them were established on the basis of spectral data including UV,IR,MS,¹H-NMR,¹³C-NMR,DEPT,HMBC,HMQC,COSY,TOCSY,NOESY , physical and chemical properties, as follows: BW-1:β- sitosterol BW-2:β- daucosterol BW-3: stigmasterol BW-4: azelaic acid BW-5: 4-hydroxyacetophenone BW-6: 2, 4-dihyroxyacetophenone BW-7: palmitic acid BW-8: n-octadecily acid BW-9: 4-hydroxybenzenemethanol BW-10: benzoic acid...