Research On Anti-HBV Effects Of PMPA

Hepatitis B is one of the major harmful diseases to human health, there are about 400 million hepatitis B carriers in the world. But so far, there is no drug to treat hepatit B fully. Chemotherapy treatment of hepatitis B is the principal means in the market now. The treatment of human hepatit B drug is mainly rely on imports or imitation in china. there is no significant effect with independent intellectual property rights of original.In the trials, propan-2-yloxymethylpho-sphonic acid(PMPA) in vitro 2215 cells inhibited hepatitis B virus(HBV) effect and in the duck animal models inhibited duck HBV effect. The cell line is from human hepatoma cell line HepG2 that is plasmid transfected HBV DNA,which can perspire HBsAg, HBeAg and the integrity HBV pellet to the liquid stably. It is an important model for studying hepatitis B virus trials.Knowing cell toxicity is through drug toxicity tests, and determine the largest of the drug dose of 20μg/ml, cell survival rate was 90.01%.Detecting HBV its inhibition is through the ELISA method, when its concentration is 80μg/ml, the rate of HBsAg inhibition reaches to 42.21%,and HBeAg inhibition rate reaches to54.91%. when its concentration is 5μg/ml, the rate of HBsAg inhibition reaches to 30.13%,and HBeAg inhibition rate reaches to31.41%. So that shows, PMPA has certain curative effect to the HBV.Trial 2 though six groups of infected ducks were treated with saline,Lamivudine and PMPA of 7.5,15,30mg/kg doses respective.Blood was collected from the duck on the day before treated ,1 week,2 week and 3 week during treatment and on 3 d,1 week after drugs were stopped.Serum DHBV DNA levels were detected by PCR and ELISA.The detection of hepatitis B indicators reflect the AFP, total protein, albumin. All results show that the drug for the treatment of hepatitis B virus with some success. Through the completion of the experiment we know, PMPA inhibit DHBV and HBV that is certain extent. PMPA has certain antiviral effect in duck body after continuously taken The experimental goal will provide the basis for this medicine later clinical practice. In order to seek the treatment hepatitis B virus medicine that is to provide the rationale.