Expermental Study Of The Effect Of Granulocyte Colony Stimulate Factor For Acute Myocaridial Infarction

ObjectiveTo establish the animal model with acute myocardial infarction to investigate the effects of G-CSF and the possible mechanism in swin model with acute myocaridial infarction.MethodsAcute myocardial infarction model was induced by block of left anterior descending coronary artery(LAD) by balloon dilation. 12 pigs were divided into the treatment and control group randomly. G-CSF(10μg/kg/day, once a day)was injected subcutaneously from 24 h after AMI for 5 days in treatment group. The control group was given saline as the same volume as G-CSF. The numbers of circulating white blood cells were counted before and at 1, 3, 5, 7, and 28 days after MI. Peripheral blood mononuclear cells was isolated by centrifugation using Ficoll of a density of 1.077g/ml. Labeled with Brdu, cells transplantation into infarcted area through intracoronary way(LAD). The control group was given saline. The animals were killed after 4 weeks. Some specimens were harvested and stained with HE. Transplanted cells and capillary density can be identified by immunohistochemical examination. Echocardiographic studies were performed after infracted myocardium in day 1 and 4 weeks.ResultsAcute myocardial infarction model could be established by technique of chronic coronary artery occlusion at a selected location with use of balloon inflation in porcines. Survival cells labled with BrdU could exhibit cardiomyogenic phenotypes in infracted cardiac microenvironment. Vascular density were significantly greater in infarct zone in treatment group than that in control group. The improvement in LVEF were greater in G-CSF group than control group in 4 week.ConclusionA swine model of AMI could be established by coronary occlusion with angioplasty balloon. G-CSF could improve heart function and stimulate angiogenesis.