Study Of Huang Qi Injection On Proliferation And Expression Of Vascular Cell Adhesion Mokcule-1 And E-selectin In Endothelial Cells

Objective: To investigate the effect of Huangqi injection on proliferation and expression of vascular cell adhesion molecule and E-selectin in human umbilical vein endothelial cells(HUVEC).To study the mechanism of Huangqi injiection on regulation of hematopoiesis.Methods:(1) Human umbilical vein endothelial cells culture model was founded,Endothelial cells was identified by immunohistochemical staining and transmission electron microscope (2)Treating HUVEC with various concentration of Huangqi injiection (0ug/ml,4ug/ml,40ug/ml,400ug/ml,4000ug/ml),Analyzed the proliferation of HUVEC by MTT; Analyzed the proliferation phase of HUVEC using flow cytometry; Measure the expression of VCAM and E-selectin by ELISA.Results: (1) The results of immunohistochemical staining on correlation antigens of VIII factor and Weible-Paladabody in the endothelial cells was observed by using transmission electron microscope.demonstrat that we established the human umbilical vein endothelial cells culture model.. (2)Every groups has different influences on cell growth(Time:P=0.000<0.05;Concentration:P<0.05).(3) There was significant change was found between the experiment group and the control group on the proliferation phase of HUVEC(P<0.05).The number of cells in S phase and G2/M phase were increased, significant(P<0.05). The number of cells in G0/G1phase were reduced . (4) Endothelial cells were cultured in vitro can secret VCAM-1 no stimulation . Different Huangqi injection and TNF can promoted the secretion of VCAM-1 from endothelial cells .400μg/ml Huangqi injection is the most proper concentration. ( P < 0.05 ) ,meanwhile, Huangqi injection can promoted the roles of TNF ( P <0.05).(5)Endothelial cells were cultured in vitro can not secret E-selectin no stimulation. Huangqi injection can not promoted the secretion of E-selectin from endothelial cells directly. Endothelial cells can been activated by TNF , Huangqi injection can promoted the secretion of E-selectin from activated endothelial cells(P<0.05).Conclusions:This research suggest that , Huangqi injection can support hematopoiesis through endothelial cells and adhesion molecule , Huangqi injection may promote the homing of hematopoietic stem/progenitor cells. Theory basis was afforded for the clinical application of Huangqi injection through this research.