Expression And Significance Of HMGB1 MRNA In Endometrial Cancer

OBJECTIVE: To investigate the differential expression of high mobility group box-1 protein (HMGB1) in endometrial cancer,atypical hyperplasia endometrium and normal endometrial tissues, and to discuss the potential role of HMGB1 in pathogenesis and development of endometrial cancer.METHODS: The mRNA expression of HMGB1 gene in 27 cases of endometrial cancer, 7 cases of atypical hyperplasia endometrium, and 13 cases of normal endometrial tissues was examined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). And the HMGB1 mRNA expression was compared in endometrial cancer tissues with different clinicopathological data.RESULTS: The expression of HMGB1 mRNA in endometrial cancer (0.3406±0.0354) was higher than that in normal endometrial tissues(0.2208±0.0170) (P<0.05); the expression of HMGB1 mRNA in atypical hyperplasia endometrial tissues(0.2316±0.0392) was higher than that in normal endometrial tissues(0.2208±0.0170),but there was no significant difference(P>0.05). The expression of HMGB1 mRNA in endometrial cancer has a correlation with clinical-surgical stage (p<0.05), metastasis of lymphnodes (p<0.01), myometrial depth (p<0.05), the patients' menses (P<0.01) and ER, PR expression (p<0.05). There was no correlation between HMGB1 gene and patients' age, cell differentiation or pathological change extension (P>0.05) of endometrial cancer. There was no correlation between HMGB1 gene expression and different menses phase of normal endometrial tissues. The expression of HMGB1 mRNA in normal hyperplastic endometrial tissues (0.2129±0.0180) was higher than that in normal secretory endometrial tissues (0.1942±0.0099), but had no significant difference (P>0.05).CONCLUSIONS: HMGB1 mRNA showed up-regulation in endometrial cancer tissues, and had a relationship with clinical-surgical stage, metastasis of lymphnodes, myometrial depth, patients' menses and ER, PR expression. It suggested that HMGB1 may have some relationship with the carcinogenesis and development of endometrial cancer, and may be related with cell proliferation, metastasis and ER, PR expression.