Effect Of Intensive Insulin In The Therapy On Serum Inflammatory Factors In DM Rats

Objective:To explore the effect of intensive insulin in the therapy on serum inflammatory factors in diabetes mellitus rats and provide a new method to therapy DM effectively.Methods:48 Wistar rats were divided randomly into two groups,32 rats in normal control group and 16 rats in diabetes mellitus group.The subjects in diabetes mellitus group were injected intraperitoneally with dose STZ 50mg/kg while the ones in normal control group were injected intraperitoneally with the similar dose of citric acid buffer.16 rats in diabetes mellitus group were randomly injected intraperitoneally with the dose of insulin 4～6U/d.At 2,4 weeks, fast Blood glucose were observed,serum TNF-α,IL-1β,IL-6 were measured by RIA.We compare these data with analysis of variance,and analyse the correlation of TNF-α,IL-1βand IL-6 in DM.Meanwhile,the pathological changes of pancreas were examined using light microscope and electron microscope.Results:Compared with the normal control group,diabetic rats expressed more higher in serum TNF-α,IL-1β,IL-6(p＜0.01)and there was a significant positive correlation between the expression of TNF-α,IL-1βand IL-6 in DM group(p＜0.05);with the DM group,the pancreatic islet in the insulin group retained a constitutive eumorphism by light microscopy and the quantity ofβcells increased with good defining power granulation,with little free ribosome, with better chondriosome and endoplasmic reticulum.Conclusion:There was a significant positive correlation between the expression of serum TNF-α,IL-1βand IL-6 in DM and the expression may play a important role in the pathogenesy of DM.Insulin may play a protective role in the therapy on serum inflammatory factors in diabetes mellitus.