Expressions Of MMP-7, TIMP-1, E-CAD And Syndecan-1 Correlation With Invasion And Metastasis In Gastric Carcinoma

Objective Adhesion and degradation are the two committed steps of cancer cells' infiltration andmetastasis. Matrix metalloproteinase-7 (MMP-7) and tissue inhibitor of metalloproteinase-1(TIMP-1) are relevant to the degradation of extracellular matrix (ECM). Eptithelial cadherin(E-CAD) and Syndecan-1 (CD138) mediate the adhesion of cell-ceU or cell-matrix. It is foundthat MMP-7 can destroy the junction of cells by degradating E-CAD. And there is a synergisticeffect between the expressions of Syndecan-1 and E-CAD. Besides, Syndecan-1 can alsosuppress the expression of MMPs. We detect the expressions of MMP-7, TIMP-1, E-CAD andSyndecan-1 in 36 gastric carcinomas by immunohistochemistry for studying the relationshipbetween expressions of MMP-7, TIMP-1, E-CAD, Syndecan-1 and the invasion, metastasis ingastric carcinoma. It also can evaluate the relationship between MMPs and cell adhesion ingastric carcinoma and improve the diagnosis and treatment of gastric carcinoma. Even it canprovide information for clinical application of TIMPs in the prevention and cure of gastriccarcinoma.Methods Streptvidin-peroxidase immunohistochemistry technique (SP) was used to detect theexpressions of MMP-7, TIMP-1, E-CAD and Syndecan-1 in 36 gastric carcinoma specimens and36 gastric mucosa membrane beside carcinoma specimens. The relationship between theexpressions of MMP-7, TIMP-1, E-CAD and Syndecan-1 in gastric carcinoma and invasion,metastasis, clinicopathological features of gastric carcinoma was analyzed statistically.Results 1. The positive expression rate of MMP-7 in gastric carcinoma and mucosa membranebeside carcinoma were 58.33%, 5.56% respectively. The positive expression rate of MMP-7 ingastric carcinoma was higher than that in gastric mucosa membrane beside carcinoma (P＜0.01).The positive expression of MMP-7 was significantly correlated with TNM staging (P＜0.01) andlymph node metastasis (P＜0.05), but uncorrelated to age, sex, tumor size, intravascularinvolvement, lymphatic vessel invasion, infiltration depth, and differentiation (P＞0.05). 2. Thepositive expression rate of TIMP-1 in gastric carcinoma and mucosa membrane beside carcinomawere 44.44%, 50.00% respectively. The positive expression rate of TIMP-1 in gastric carcinomawas lower than that in gastric mucosa membrane beside carcinoma, but it was not significantstatistically (P＞0.05). The positive expression of TIMP-1 was significantly correlated with TNM staging (P＜0.01), but uncorrelated to age, sex, tumor size, intravascular involvement, lymphaticvessel invasion, infiltration depth, lymph node metastasis and differentiation (P＞0.05). 3. Thepositive expression rate of E-CAD in gastric carcinoma and mucosa membrane beside carcinomawere 72.22%, 91.67% respectively. The positive expression rate of E-CAD in gastric carcinomawas lower than that in gastric mucosa membrane beside carcinoma (P＜0.05). The positiveexpression of E-CAD was significantly correlated with differentiation (P＜0.05), but uncorrelatedto age, sex, tumor size, intravascular involvement, lymphatic vessel invasion, infiltration depth,lymph node metastasis and TNM staging (P＞0.05). 4. The positive expression rate of Syndecan-1in gastric carcinoma and mucosa membrane beside carcinoma were 55.56%, 86.11% respectively.The positive expression rate of Syndecan-1 in gastric carcinoma was lower than that in gastricmucosa membrane beside carcinoma (P＜0.01). The positive expression of Syndecan-1 wassignificantly correlated with lymph node metastasis and infiltration depth (P＜0.05), butuncorrelated to age, sex, tumor size, intravascular involvement, lymphatic vessel invasion,differentiation and TNM staging (P＞0.05). 5.18 in 36 cases of gastric carcinoma were positivelyexpressed both E-CAD and Syndecan-1 and 8 in 36 cases were negatively expressed bothE-CAD and Syndecan-1. It indicated strong relationship between the expression of E-CAD andSyndecan-1 (P＜0.05). But the relationships of MMP-7/TIMP-1, MMP-7/E-CAD andMMP-7/Syndecan-1 were not significant statistically (P＞0.05).Conclusion 1. The positive expression rate of MMP-7 in gastric carcinoma was higher thanthat in gastric mucosa membrane beside carcinoma. The positive expression rates of E-CAD andSyndecan-1 in gastric carcinoma were lower than that in gastric mucosa membrane besidecarcinoma. 2. The positive expression of MMP-7 was significantly correlated with TNM stagingand lymph node metastasis. The positive expression of TIMP-1 was significantly correlated withTNM staging. The positive expression of E-CAD was significantly correlated with differentiation.The positive expression of Syndecan-1 was significantly correlated with lymph node metastasisand infiltration depth. 3. The correlative expression between E-CAD and Syndecan-1 in gastriccarcinoma indicates that both of them play an important role in infiltration and metastasis ofgastric carcinoma cooperatively. 4. Combinative detection of MMP-7, TIMP-1, E-CAD andSyndecan-1 expressions in gastric carcinoma may be valued to recognize the mechanism oftumor invasion and metastasis, to evaluate the clinical progress of gastric carcinoma and to provide scientific basis for anti-angiogenesis in treatment of gastric carcinoma.