Expression And Singnficance Of Proto Oncogone C-fos And C-jun In Vulvar Squmous Cell Cacinoma

Purposesurgical operation and live issues check from January, 1995 to JanuaryCurrently, the physiology function of proto oncogene causing value more and more. C-fos and C-jun are the cancer genes which code to check nuclear protein, belong to the early gene.This kind of gene is subjected to immediately express by various incitement and produce to transcript factor.This kind of gene adjust to control transcription and expresstion of other geneses and regulate the growth, development and division of normal cell. Expression outcome of Jun and the Fos oncogene are located in the cell nucleus. Currently have already know 3 kinds of Jun proteins (C-jun, JunB and JunD) and four kinds of Fos proteins (C-fos, FosB, Fra-1 and Fra-2).They participate composing transcribe and activate factor(Ap-1) and regard the C-jun and the C-fos as principle among them. C-fos and C-jun form same source or different source dimmer through leucine zipper structure and have transcribe activation. Therefore is as well as called transcribe factor (Ap-1).After AP-1 that C-jun and C-fos dimmer combine with DNA, can regulate the the expression of downstream target gene in transcribe level, and participate cell propagation and convertion. The research expresses that Ap-1 is related to cell propagate, having key fuction in conversion of tumor and converse. Mankind C-fos oncogene is the same source of V a fos gene inside the cell that the rat FBJ2 bone tumor is converses transcribed.The fixed position is at the chromosome 14 qs 23.4, and its outcome C-fos has 380 amino acidses. Mankind C-jun oncogene is the same source of ASV17 inside the cell that the birds tumor is converses transcribed. The fixed position is at the chromosome 1 p 322 ps 31. The protein outcome has 331 amino acids. C-fos and C-jun protein have no function alone. Jun protein must become a same source dimer or become a different source dimmer with C-fos proteinand and ATP protein. The latter regulate the signal that thoroughfare upper stream spread. The outside incitement is changed the start thing or to build up thing inside and have the particular gene expression through AP1 combine point. The different sourcedimer that C-fos and the C-jun form can combine DNA component of 5' TGAGTCA 3'. The AP1 is transcribe factor,and identify a particular DNA sequence inside the cell, having transcribe and regulate activity, then influence particular target gene inside cell and promote cell growth and divide, induce tumor occurrence. Currently, Study to discover: the C-fos and the C-jun oncogene are relevant with occurrence, development and prognosis of tumor. In the stomach cancer, lung cancer, carcinoma of cervix, skin squamous cell carcinoma, protein expression of C-fos and C-jun increase. But in vulvar squamous cell carcinoma how to express still don't know, therefore, this study inspects how to expression about C-fos and C-jun in vulvar squamous cell carcinoma and Vular intraepithelial neoplasia,also analyze the relation with vular carcinoma.MethodsSelect wax lumps were cutted off by, 2005 at the Chinese medicine department university the first hospital pathology room, VSCC30 example;(vulvar squamous cell carcinoma)VIN40 example, among them, the VINⅠ—VINⅡ20, the VINⅢ20 example; 10 vular normal skins to check against. Adopt the immunity organization S-P method. Examine expression of C-fos and the C-jun protein in different pathologic classification and clinical stage of vulvar squamous cell carcinoma. Contrast to vular intraepithelial neoplasia and normal issues, and compute their ratioResultsThe C-fos has no expression in normally skin and the VINⅠ—Ⅱand 13.6%positive cell count in the VINⅢand 30.2% in VSCC. compare to show notable difference(p＜0.05). The C-jun has no expression in normally skin and the VINⅠ—Ⅱand 18.5% positive cell count in the VINⅢand 40.6% in VSCC compare to show notable difference.(p＜0.05). C-fos and the C-jun protein expression decline gradually along with vulvar squamous cell carcinoma clinical stage go up, pathologic classification fall and lymphatic knot transfer(p＜0.05). Two kinds of cancer gene protein C-fos and C-jun contain stronger relativity.ConclusionThe oncogene C-fos and C-jun have close concern in occurrence and development of vulvar squamous cell carcinoma. C-fos and the C-jun protein expression decline gradually along with vulvar squamous cell carcinoma clinical stage go up, pathologic classification fall and lymphatic knot transfer(p＜0.05). Two kinds of cancer gene protein C-fos and C-jun contain stronger relativity.