Expression And Significance Of Survivin In Bladder Transitional Cell Carcinoma

BackgroundBladder transitional cell carcinoma(BTCC), possessing complicated biological behavior, is the most common malignancy carcinoma in the genitourinary tract in China. With the long average natural life and the environment pollution caused by industry and agriculture development, the incidence of the BTCC is increasing rapidly. It's the second incidence in the malignancy carcinoma in genitourinary tract in the world and the first incidence and death rate in China. It has brout heavy economic burdens to patients and their families. In order to improve the overall survival rate of bladder cancer patients, scientists have made large and increasing interest on searching markers with diagnostic and/or prognostic value. Recent years, lots of studies have been done on the role of survivin in neoplasm including BTCC. Survivin is a member of the inhibitor-of-apoptosis proteins(IAPs) family, commonly detected in tissues during fetal development and in cancers, but not usually in adult normal tissues. Survivin protein has the ability to suppress apoptosis(programmed cell death) and regulate cell division. Expression of survivin protein and its mRNA may be used for diagnostic and/or prognostic indicator in many cancers. In order to know more about the Suvivin and the relationship between the incidence development and prognosis of BTCC, the author made some research. The enzyme linked inmunosorbentassay (ELISA)/RT-PCR/immunohistochemical method were used to detect the survivin protein in urine/survivin mRNA in urine exfoliated cells/survivin protein in carcinoma and normal tissues. Perhaps the results may bring a new approach for gene diagnoses and therapy of BTCC.Methods1. Collection of the clinic cases and normal cases: The urine and carcinoma tissues were brought out and saved for experiment.2. Detection of survivin protein in the urine: The survivin protein were detected by enzyme-linked inmunosorbentassay (ELISA) method, and the data was recorded and analysed.3. Detection of the survivin mRNA in urine exfoliated cells: The RT-PCR method was used to detect the survivin mRNA in urine exfoliated cells.4. Assay of the survivin protein in carcinoma and normal tissues: The sections of the tissues were subjected to survivin immunohistochemical reaction with SP method. The result was recorded and the data was analysed.Results1. The urine survivin protein in the BTCC group was significant more than that in the control group (p＜0.05).2. The urine survivin mRNA positive rate in the BTCC group was also higher than that in the control group (p＜0.05).3. Density of survivin protein immunohistochemical reaction in BTCC group was more dense than one in the control group. The positive rate in BTCC group was also higher than that in the control group (p＜0.05).Conclusion1. The RT-PCR examination for assay of urinary exfoliated cell survivin mRNA is a sensitive one to BTCC. So it can be uesed as a assistant diagnosis of BTCC..2. There is no survivin protein in the normal bladder mucosa. But there's a strong expression in the carcinoma. So the expression level of survivin protein can be used as an assistant diagnosis index.3. With the development of BTCC in clinical and pathologic assay, the survivin protein expression was more and more stronger. There's a high relativity between the suivivin expression and the bladder carcinoma.