| Objective: Mesangial Proliferative Glomerulonephritis (MsPGN)is the common pathological type of diverse renal glomerular disease. According to the findings of epidemiological investigation of China, incidence rate of primary glomerulonephritis is 76.8%~81.7%in primary glomerulopathy, and MsPGN is 29.7%~59.6%. So MsPGN is one of the main reasons leading to renal failure. Now we always treat it by excitatory autacoid,Immunodepressant. But the drugs of their categories always accompanied with obvious adverse reaction. So if we can find some good curative effect and less adverse reactive drugs with multiple therapeutical effects, it will can play a very important roll in the treatment of kidney diseases. Complex prescriptive Chinese medicines with multiple therapeutical effects have particular ascendancy The complex prescriptive Chinese medicine—shenshukang(SSK) is a good curative effect empirical formula that we combine according to characteristic of chronic glomerular nephritis.In this study, model of mesangial proliferative glomerulonephritis(MsPGN) was made with immunization.The MsPGN rats were cured by ShenShuKang (SSK). Proteinuria plasma protein, blood-fat, renal function, renal pathomorphology, the expression of renal TNF-αwas observed. The action and mechanism of SSK on MsPGN were investigated so as to provid experimental data.Method : Thirty-six male Sprague-Dawley rats were randomly divided into four groups: Normal, Model, SSK and TW group. 24h urine protein was determined with the trichloroacetic acid turbidimetry. SerumTP, Alb, TC ,TG , HDL , LDL,BUN,Scr were assayed with the automaticbiochemistry analyzer. Each group of the mesangiu area and the membrane cell (GMC) and the extracellular matrix (ECM) were observed with light microscope, electron microscope. The expression of TNF-αwas observed with the immunohistochemical staining. The section of HE, Masson, PAS staining and immunohisto- chemistry staining was analyzed with the pathology image analysis system.Results:(1) 24h urinary protein quantitative analysis showed that: At the 2nd, 4th weekend, the excretion of urinary protein in every group was not increased markedly. At the 6th, 8th, 12th weekend, the excretion of urinary protein in Model group was higher than Normal group's (P<0.01). The two treatment group, compared with Model group, at the 6th weekend, 24h urinary protein quantitative were descent, but there was no significant difference between them (P>0.05); At the 8th weekend, in two treatments groups, urine protein quantitative were descent remarkablly, compared with Model group and there was remarkable difference (P<0.05), At the 12th weekend, Model group urine protein compared with two treatment groups, had the unusual significance difference (P<0.01), SSK group urine protein compared with TW group, at the 6th, 8th, 12th weeks had no statistics significance (P>0.05).(2) At the 12th weekend each group of biochemistry analysis showed that: TP and Alb of the blood serm in Model group was descent significantly than Normal group (P<0.01); Meanwhile, TP and Alb in two treatment groups increased significantly than Model group (P<0.05). There was no significant difference between two treatment groups (P>0.05). TC, TG and LDL of the blood serm in Model group increased significantly (P<0.01), but HDL was decented markedly (P<0.01); TC, TG and LDL of the two treatment group was decent than Model group (P<0.01), but HDL was increased markedly (P<0.01), there was no significant difference between the two treatment group (P>0.05). BUN and Scr of the blood serm in Model group were higher than Normal group significantly (P<0.01); BUN and Cr of two treatment groups decented significantly than Model group (P<0.01), but there was no significant difference between the two treatment groups (p>0.05).(3) Nephridial tissue pathomorphology showed that: In model group, the mesangial region expanded obviously, matrix increased, blood capillary narrowed or disappeared and index of mesangial matrix increased; Compared with the model group, the above pathology change of two treatments groups obviously reduces, matrix index remarkable dropped (P<0.01), blood capillary were obviously improved, There were no significant difference between the two treatment group (P>0.05).(4) In nephridial tissue, immunohistochemistry and RT-PCR result of TNF-α: Compared with Normal group, the expression of TNF-αwas obviously enhanced (P<0.01) in the Model group. Compared with Model group, the expression of TNF-αwas diminution (P<0.05, P<0.01), in two treatments groups.There was no statistics significance between the two treatment group (P>0.05).Conclusions:1 SSK can reduce MsPGN rat's protein urine, increase plasma protein, improve lipid metabolism, decrease BUN and Scr to protect renal function.2 The SSK can refrain MsPGN rat's MC proliferation, reduce ECM deposition and delay the process of MsPGN. The mchanism was that SSK has the effect to inhibit the expression of TNF-α.3 SSK maybe be similar to TW in the effect of treating MsPGN.
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