Genetic Polymorphisms Of CYP1A1 MspI Associated With Susceptibility To Esophageal Cancer In The Kazakans

Object: The study about the gene porlymorphism of the metabolishment enzyme andthe esophageal cancer susceptibility have been gradually become the hot spot in the field ofthe tumour molecular epidemiological study. In this study we investigated the relationship ofthe CYP1A1MspI porlymorphism and the susceptibility of esophageal cancer in Kazakh inXinjiang.Methods: We take the Kazakans population in the YILI region where has highincidence and mortality rate of the esophageal carcinomaes as our topic investigation object.The study included 92 new cases of esophageal cancer and 184 controls (1:2) which collectedfrom the same area. Blood samples were collected from all cases and controls, and PCR-RFLP was use to analyze the CYP1A1MspI polymorphisms. The relationship between theheredity porlymorphism of CYP1A1MspI and esophageal carcinoma susceptibility, the datewas analysed with statistics software of the SPSS 11.0. The odd ration (OR) and the 95%confidence interval (CI) represent the relative risk. We analyse the 276 samples' geneporlymorphism (including 184 normals and 92 patients) and discuss of the Kazakanspopulation. At last we analyse the data and verify the samples' population representativenesswith the Hardy—Weinberg equilibration law. The x~2 test was adopted to check the differencein genotype distribution between patients and controls, and the accordance withHardy-Weinberg equilibrium in the group.Results: (1) The frequencies of TT, TC and CC in the CYP1A1MspI porlymorphismwere 28.26%,55.43%,16.30% in Kazakh's individuals with EC, respectively. They were32.07%,51.09% and 16.85%。in controls individuals x~2 =0.52, P=0.77, P＞0.05, there is nostatistical difference between esophageal cancer patients and controls. (2) The testers weredivided into two groups. The male frequencies of TT,TC and CC were 30.0%,51.7% and18.33% in Kazakh's EC and 36.4%,47.7% and 15.9% in controls, respectively, nosignificant difference between them was found, x~2=0.2, P=0.91, P＞0.05.The femalefrequencies of TT,TC and CC were25.00%,62.50% and 12.05%,in Kazakh's esophagealcancer and 35.94%,45.31% and 18.75% in controls, respectively, no significant differencebetween them was found, x~2=2.53, P=0.28, P＞0.05,(3)In order to estimate the risk factor,we divid the experiment object into 7 groups according to the age (＜40 years;＞70years).Nosignificant difference was found in male group, x~2=1.56, P=0.46, P＞0.05, the same resultwas observed in female groups, x~2=1.79, P=0.41, P＞0.05. (4) CYP1A1MspI allele distribution:the mutation pure allele in patient group 44.0% and contrast group 40.1%, there was nostatistical significance (P＞0.05). (5) Hardy-Weinberg equilibrium test was performed in thegropes and the result showed no statistically significant difference(x~2=0.52, P=0.77,P＞0.05).Conclusion: The polymorphisms of Cytochrome P450 enzyme CYP1A1 MspI geneis not related with the genetic susceptibility of Esophageal cancer in Kazakh.