Association Research Between Environmental Risk Factors, Polymorphism Of MTHFRC677T And Susceptibility To Esophageal Cancer In Xingjian Kazakh

Objective: This study is on the basis of epidemic investigation of behavior factor of about esophagus cancer(EC)patient and the comparison crowd in Kazakh in Xinjiang Kazakh, To investigate and the polymorphism of methylenetetrahydrofolate reductase (MTHFR) C677T ,discussing the connection between Kazak EC patient and behavior factor, polymorphism of MTHFRC677T.Method: Using 1:2 matched case-control study, we have identified 92 esophageal cancer patients with endoscopy and pathology diagnosis from hospital in north of Xinjiang during the periods from March, 2005 to March, 2006.A total of 184 controls without history of malignant tumor or digestive system diseases were selected during the same period as cases and matched to each case by gender, age, nationality and inhabited areas. The HinfⅠpolymorphism of MTHFR677 was detected by PCR-RFLP and applying chymist radiation measure folate content in plasm. Statistical analysises adoptχ2 test, conditional logistic regression to calculate the values ofχ2, odds ratios and 95% confidence intervals for possible risk/protective factors. All the calculations and statistics were performed with the computer program, SPSS Version 13.0. Check the accordance with Hardy-Weinberg equilibrium in the group.Result: 1. 1:2 matched case-control study analysis showed: not sanitation of drinking water, drinking wine, liking to eat peppery food, fast eating, irregular eating time ,the history of stomach or esophagus illness and family history of EC. EC patient folate content 3.373±1.43ng/ml,controls 3.960±2.05ng/ml,Folate content of EC patient is different form of controls group.(t=-2.389,p=0.018)2.The frequencies of CC,CT and TT were 70.7%,24.5%and 4.7% in Kazakh's controls and 52.2%,43.5% and 4.3% in Kazakh's individuals with EC, The genotype frequencies of EC group is different form of controls group (χ2=10.492,P<0.05). The individuals with 677C/T genotype had a 2.537-fold (95%CI: 1.432～4.494) increased risk of developing EC compared with those who had 677C/C genotype. The allele T of MTHFR had a 1.709-fold (95% CI : 1.115～2.167) increased risk of developing EC compared with those who had allele C.3.Multivariate conditional Logistic regression analysis showed: not sanitation of drinking water ,a liking to eat peppery food , fast eating , fresh vegetable and fruit lacking ,the history of stomach or esophagus illness and family history of EC were important risk factors of EC. The population attributable risk of the five factors was 18.6%, 9.5%,15.7%,72.89%,13.6% and 7.6%.4.The interaction between folate content and MTHFRC677T were results:when the individuals are deficient folate in body,its can increase fatalness with MTHFR677C/T,T/T genotype crowds.Conclusion:(1)It could increase incidence of esophageal cancer in condition of drinking water and bad diet manner, to eat fresh vegetable and fruit seldom in Kazakh Xinjiang in Kazakh.(2)Folate lacking or polymorphism of MTHFRC677T were related with esophageal cancer in Kazakh in Xinjiang.(3)Interaction between gene and environment showed that:folate lacking and polymorphism of MTHFR C677T decreased risk factor of esophageal cancer in Kazakh in Xinjiang.