Study On Chemical Constituents Of Ixeris Sonchifolia(Bge.) Hance

Bitter saucer is the dried full grass of Ixeris sonchifolia(Bge.)Hance.There are more than 50 species all over the world ,it is mostly distributed at China , Korea , Japan , Vietnam , Ussr and so on . there are 20 species in our country . It is abundantly distributed throughout southern and eastern mountainous area of the northeastern china and xiaoxinganling region . the chemical composition of bitter saucer is complicated , it mostly contain adenosine , flavonoid , sesquiterpenes lactone and triterpene saponin , added to this ,it also contain sterol , coumarin ,wood fat white , vitamin , amino acid , sugar and so on . According to〝mongolia Chinese herbal medicine〞record,it is bitter in taste, cold in nature ,possesses the efficacy of clearing away heat and toxic material , removing heat from the blood , promoting blood cieculation and drainage . It is used to cure appendicitis ,enteritis , headache , toothache , and so on of the folks . Its primary pharmacological action are resisting tumour , antiphlogistic , analgesia , mitigation and activity of cardiovascular .Therehave be bitter saucer injection and diemailing injection using in clinic .In recent years , with the development of modern extraction separation , spectroscopy technology and modern pharmacology , the research ofchemical composition and biological activity of Ixeris sonchifolia is moreand more indepth .But it focus on liposoluble part mostly,it's application is primarily water soluble injection . wherefore wo should increase the research of its water soluble part .Bitter saucer is lift by water and fathomed by alcohol ,deposited bycalcium oxide . Availing solvent extraction , macroporous occluded resin, polyamide column chromatography , MDS reversed-phase preparated chromatography and half-preparation HPLC chromatography of instrument, glycosides were discriminated through laminae , saccharics were discriminated through GC . five compounds were separated from the extract of Ixeris sonchifolia . On the basis of spectral analysis and literature comparison , these compounds were clucidated as luteolin-7-O-β-D-glucopyranoside (Ⅰ) ,luteolin-7-O-β-D-glucuro-nopyranoside (Ⅱ),Apigenin-7-O-β-D-glucopyranoside (Ⅲ) , chicoric acid (Ⅳ) , luteolin-7-O-β-D-glucopyr-anosyl-(1→2)-β-D-glucopyranosid (Ⅴ) . Among of them , the compoundⅣis the first time to be get from the plant .Pharmacological experiment indicated that the extract of Ixeris sonchifolia possessed effective myocardium protective action ,pharmacological action of compoundⅡis more prominence (P<0.001) , compoundⅢandⅣhave some pharmacological action (P<0.05) , the match of the bothcompoundⅠandⅡhave obvious protective action to myocardial cell .