| Diabetes mellitus(DM)is a chronic metabolic disorder. We also known as type 2 diabetes mellitus(T2DM). T2DM accounts for 90%-95%of allthe diabetes patients.Recent years the incidence of DM has been growing rapidly in China with a current number of diabetes patients over 30 million. The most dangerous factor of fatality and mutilation is the macroangiopathy. If it is happened, the mortality will increase apparently. Some tests demonstrate that the rate of macroangiopathy is two times in patients who have Diabetes mellitus than normal people. That will take suffering for the patients, and also need more money , it become the severe ecnomic burden to our society. So it is urgent to identify the etiology of DM and how to prevent it. As a indispensable factor of the thrombosis, the platelet paticepate the process in making artherosclerosis and making thrombosis, it also influence the vasospasm. Platelet glycoprote(Gp) has the relationship with the adhesion and aggregation of the platelet. So the number and the density of the Gp is the molecular basis for inhancing the activity of the platelet. Thrombosis is controled by variety Platelet membrane receptor complex. The GPⅡb/Ⅲa complex is the most important, it also the target of the antiplatelet drug. So it has the significance to prevent and heal the ischemia which is made by thrombosis. There are many tests about the gene polymorphism of GPⅡb/Ⅲa complex. Our purpose is to know the distribution of GPⅡb/Ⅲa complex gene polymorphismin in Chinese people and the relationship with the Diabetes mellitus with cerebral infarction(CI). As the most documents in the china certify GPⅢa has no apparent gene polymorphism in chinese people, we only research the gene polymorphism of the GPⅡb.Subject and Methods1.SubjectDM with CI group: 100 objects come from Jilin University First Clinical Hospital. All of them coincidence the diagnosis of type 2 diabetes mellitus accorded with the new diagnostic criteria of WHO in 1999. As the same time, all of them also coincidenc the diagnosis of cerebral infarction was defined according to the CT. There are 52 women and 58 men in this group. The average age is 60.7士7.6.DM group: 100 objects come from Jilin University First Clinical Hospital. All of them coincidence the diagnosis of type 2 diabetes mellitus accorded with the new diagnostic criteria of WHO in 1999. There are 31 women and 69 men in this group. The average age is 60.5士9.3.Control group: 100 objects are not CI nor DM. There are 31 women and 69 men in this group. The average age is 58.7士10.2.All the objects have no kinship.2.Criteria of diagnosis:(1) The diagnosis of type 2 diabetes mellitus accorded with the new diagnostic criteria of WHO in 1999. (2)The diagnosis of cerebral infarction was defined according to the CT.3. Laboratory methods for GPⅡb gene polymorphism(1)DNA was extracted from the peripheral blood leukocytes by standard phenol and chloroform method.(2)GpⅡb genotype was determined by PCR-RFLP, the PCR product were separated on 2% agarose gels and visualized with ethidium bromide.4.statistic analysisUsing the SPSS13.0 for windows statistical package Count Data were performed by Student's-test and measure data were performed by chi-squared test. Genotype and allele frequencies between groups were analyzed by the chi-squared test. Statistical significance was taken as p<0.05.Result1. There is a polymorphism of GpⅡb in the population of northeast of China. The GpⅡb genotype distribution of the control group, DM with CI group and DM group was in Hardy-Weinberg equilibrium(p>0.05).2. The distribution of genotype in GpⅡb of all the samples has no significant in the factors of DM with CI(such as: sex, hypertension and if smoke or not)3. The distribution of genotype ab and bb in DM with CI group and DM group was significant more than in control group. The b allele frequency in DM with CI group and DM group is significant higher than in control group(x~2=21.71,P<0.01). The distribution of genotype aa, ab, bb in GpⅡb of all the samples was significant(x~2=7.55,P<0.01).4. The distribution of genotype ab and bb in DM group was not significant more than in control group(x~2=2.22,p>0.05). The b allele frequency in DM group is not significant higher than and control group (x~2=0.57,P>0.05).5. The distribution of genotype aa, ab, bb in in GpⅡb of DM with CI group and DM group was significant(x~2=8.58,p<0.05). The b allele frequency in DM with CI group is significant higher than and DM group (x~2=4.02,P<0.05).Conclusions1. There is a polymorphism of GpⅡb in the population of northeast of China.2. b allele may be a susceptibility gene of DM with CI.3. GpⅡb gene polymorphism may be not associated with DM.4. GpⅡb gene polymorphism may be associated with the factors of DM with CI(such as: sex, hypertension and if smoke or not)...
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