The Effect From Chinese Materia Medica Of Warming Kidney And Dispersing Blood Stasis On Stroma-cell Of IM In Endometriosis

Endometriosis is one of intricate disease in department of gynecology commonly, that is defined as endometriotic tissues growing outside normal uterine cavity. Although it is benign, its behavior is malignant, which has confused the clinicians. the stduy has been to emphasize on rationale of treatment by the chinese materia medica of Warming kidney and dispersing blood stasis, preparation serum of the herbs, and the initial establishment of cultural method of vitro-clearing stroma-cell. It has provided experimental model in order to study effect of the chinese materia medica of Warming kidney and dispersing blood stasis on endometriosis, and to approach its mechanism of action.1,The theoretical search of endometriosis treatment by the Chinese materia medica of Warming kidney and dispersing blood stasisThere is no morbid name about endometriosis in medical science of homeland. The cardinal symptom are correlated closely to algomenorrhea, abdominal mass and infertility etc. The disease was due to disfunction of the dominating reproduction of the kidney, overacting in sexual intercourse during menstruation and postpartum,and then lead to damage or the Chong and Ren meridians, and failure of the functions of storing and discharging of the uterus, Whether the kidney Qi was sufficient or not was the radical factor of occurrence, development of the disease, and blood stasis was the pivot of occurrence, development of the disease, so the treatment should be based upon the concept of warming reinforcing kidney and dispersing blood stasis.Guizhi fuling pill had the effect of warming kidney and dispersing blood stasis, and originally, zhongjing Zhang designed this prescription to mildly remove mass in the abdomen, disperse blood stasis in order to prevent the abortion which was due to the abdominal mass endangering the fetus, If the prescription was utilized mainly to remove blood stasis and disperse the mass in the abdomen, there might be some shortage of the strength of the drugs in the presciption, and it should be added with more drugs with the property of promoting blood circulation and resolving blood stasis. The above mentioned notion gave the theoretical foundation for vitro empirical study on endometriosis theatment by Guizhi fuling Pill Jiawei Decoction, that was formed combining the Guizhi fuling Pill with the herbs, Xianlingpi and Xuejie.2,In vitro culture method about stroma cell established initiallyThe endometria were been collected from patients suffering from endometriosis and receiving operation from April to December in 2007, which stroma-cells were been isolated, go down to the future generation, which proliferative condition were been observed by microscope, and purity coefficient of cell was be characterized by immunohistochemistry method.As a result, the proliferative condition of stroma-cells cultured out-of-body was fine, which cultural method adopts separation by grit mainly. During observation, cells are all simple interstitial cell, what was be gone down to 4-5 generation.and could take 25-30 days. It can be show from the reslut of immunohistochemistry that the purity coefficient achieve percent 95 above.It can be seen from result that the stroma-cell cultured out-of-body has satisfactory ability of vegetation, at our experiment condition. The path to draw the material and its menstrual cycle made an effect to stroma-cell's survival rate and ability of growth.( P<0.05), fine in vitro cell model can be provided to reveal pathogenesy of endometriosis and pharmacodynamics study.3,The effect on appearance of stromal-cells and IL-6 from cell culture supernatant fluid by herbs of warming kidney and dispersing blood stasisThere are serums from different dosage drugs of warming kidney and dispersing blood stasis, what interfered in stromal-cells of IM of patients sufferring EMs. Then we observed morphologic change by the inverted microscope, collected supernatant liquid of cell culture at the time of 24h,48h,72h, respectively, IL-6 were detected from supernatant liquid collected by the method of ELISA.It can be seen from morphology observation, that active suppression to stromal-cell by serums from drugs of warming kidney and dispersing blood stasis was definite initially, and what show concentration dependent; The drugs of warming kidney and dispersing blood stasis can cut down externalization of IL-6 from cell culture supernatant fluid obviously.As a result, we can presume that one of effects to control morbility and development of EM were adjusting of immunization, restrain inflammatory topo-reaction of endometria and abatemen adherence at pelvic cavity.