Studies On Quality Control And Evaluation Technique Of Glehnia Littoralis

The dried roots and rhizomes of Glehnia littoralis Fr. Schmidt ex Miquel. have been used as Traditional Chinese Medicine ( TCM ) for the treatment of respiratory and gastrointestinal disorders. Its roots mainly contain constituents such as coumarins, cournarin glycosides and polyacetylenes. It has been reported that this herbal drug showed several pharmacological activities, including stopping cough, dispelling phlegm, antitumor-promotion, antimutation, antitumor, antibacterial, antifungal, analgesic and sedative, inhibition of tyrosinase, and immuno- suppressive activities.G. littoralis is widely applied to clinic as TCM, but in actual application, there is often confusion between Glehnia littoralis and Radix Adenophorae, and there isn't detailed description on quality control in Pharmacopoeia 2005. In order to search the sciencetific methods of qulity control and processing, a systemic research of chemical constituents is carried out, resulting in 9 compounds including a new one. Of which, four components were isolated from this herbal drugs for the first time. Based on chemical studys, we developed the fingerprint and content determination methods of Glehnia littoralis by HPLC. The fingerprint included 12 mutual peaks. 2 components, panaxynol and facarindiol could be determined simultaneously by the method. In additionally, we comparatively analyzed the difference from various source drugs. The results can give some evidence for the quality control and processing of G. littoralis.1. From G. littoralis, 9 compounds were isolated and purified by silica gel, Sephadex LH-20, and HPLC chromatography methods. Their structures were elucidated depending on extensive spectroscopic analysischromatography methods. Their structures were elucidated depending on extensive spectroscopic analysis and comparison with literatures. 9 compounds were determined as lupane(1), betulin(2),β-sitosterol(3), daucosterol(4), panaxynol(5), facarindiol(6), (8E)-1,8-hepta -decadiene -4,6- diyne -3, 10 - diol(7), isoimperatorin(8) and naphthisoxazol A(9). Amomg them, 1~4 were fitstly obtained from this drugs, 9 is a new compound. It was reported that both compound 1 and 2 showed activities of stopping cough and dispelling phlegm. 2. The HPLC-fingerprint of G. littoralis, including 10 comutual peaks, was established according to analysis result of 10 samples under the optimum chromagraphic conditions, in which, 2 characteristic components were recongnized by comparison with standard compound. The area of the non-mutual peaks was less 10% than total mutual peaks', and the system suitability tests was in compliance with the fingerprint techniqe specifications.3. A simultaneous determination of falcarindiol and panaxynol in G. littoralis was developed by RP-HPLC. The established method was of good reproducibility, separation and precision, it can be used as quality control method. The content determination of two components in 23 samples showed a great difference.4. In the studying on the processing of G. littoralis, we comparatively determined the fingerprint and contents of falcarindiol and panaxynol in drugs, the results showed that both comutual peak areas and contents of two polyacetylenes were more in peeled drugs than ones. It was suggested that the processing of G. littoralis shouldn't peel the skin off the roots accoding to the fingerprint and content determination.5. The determination of falcarindiol and panaxynol in G. littoralis from commercial, cultivated and wild plant drugs showed that the content of two polyacetylene in wild plant drugs was obviously higher more than cultural one, and the same was between cultural and commercial one.