Empirical Study Of VEGF ASODN Gene Therapy Diabetic Retinopathy

Objective:In diabetic retinopathy animal model discern used VEGF ASODN(Vascular Endothelial Growth Factor Antisepses Oligodeoxynucleotides) introduction corresp. Experiment rat's eye corporis vitre ansfection retinal cells。Analysis effectiveness, To reveal VEGF ASODN down egulation retina VEGF express and, inhibition new vessels formation。To use possess high molecular's dihydroxyfluorane progress dabble opacification in rat's optomeninx,observation VEGF ASODN depressant on diabetic retinopathy rat new vessels permeability, to decrease new vessels。Methods:design two design fragment complementation at VEGF mRNA , Discern utendVEGF first second exon joining region ( ODN )。oligonucleotide( oligodeoxynucleo -tides ODN )'s base sequence for: ASODN :5'-TCCAGGGACCACTTGGCATGGTGGA-3。Shanghai shenggong biology company (business establishment) take responsibility synthesis。Selectei fifty-five health maleness rat in two month old and weight 200g (medical college experimental anima center to supply)。Adaptability feed two days,Take fifteen rats to feeding (control group C), Other forty rats, after fast 12 hours according to 60mg/kg all peritoneal injection 2%STZ solution ,use 0.5mmol/L Citric Acid balanced solution(pH 4.5) prepare before ues it,after injection 48 hour collect tail blood to measure blood sugar and urine glucose,use blood sugar.≥16.7 mmol/L,urine glucose masculine rat set to diabetes model ,in all 30。Take these diabetes rats to part breeding three moths,after experimental animal model feeding three moths trabant dividatur two classes (esch class include 15 animals),use 100umol/L VEGF ASODN inject class A corresp animal eye corpus vitreum,transfection retinal cells。Use aequales isotonic Na chloride inject class B corresp animal eye corpus vitreum After three days repeat injection one time。Each class take five rats,use high molecular dihydroxyfluorane infuse rat's heart,extirpation eyeball and fixation quickly in micro,Liberation retina and stretched preparation in fluorescence microscope ,observe retinal vessel disposition and morphousResults1,To contrast inject VEGF ASODN experimental group A retina VEGFand phase ratio control group B rat retina VEGF immunityhistochemistry staining , The former VEGF express comparatively poor,new vessels decrease。2,After observation in fluorescence microscope, it is thus evident that diabetic retinopathy rat retina effusion,blood loss,microangioma.。After inject VEGF ASODN it is thus clear that ;blood vessel morphous integrity figure appearance distinctness。ConclusionsVEGF ASODN possess a few depressant effect in STZ antileptic diabetic retinopathy rat VEGF ensemble express,decrease diabetic retinopathy new vessels formn ,accordingly amelioration diabetic retinopathy new vessels exudation。...