Effect Of Chondroitin Sulfate On Liver Fibrosis Induced By Alcohol In Mice

Objective The roles of chondroitin sulfate in liver fibrosis induced by alcohol in mice was investigated. In order to investigated the cure mechanism, the level of malondialdehyde (MDA),nitric oxide (NO),nitric oxide synthase (NOS),transforming growth factor beta 1(TGF-β1),platelet derived growth factor(PDGF),tissue inhibitor metalloproteinase-1 (TIMP-1) and collagen was detected.Methods Mice'liver fibrosis model was induced by alcohol input for 24 weeks. Chondroitin sulfate injection 25 milligram per kilogram was given intra-abdominally to the mice every day for 6 weeks. The level of MDA,NO and NOS in serum and liver homogenate were detected. Van Gieson staining detected the level of collagen in liver tissue. The level of TGF-β1,PDGF and TIMP-1 were detected by immunohistochemistry.Results Chondroitin sulfate could improve the pathological changes of liver tissue, and decreased the level of collagen (P<0.01). MDA,NO,NOS,TGF-β1,PDGF and TIMP-1 expression levels were increased in liver fibrosis induced by alcohol. However, MDA,NO,NOS,TGF-β1,PDGF and TIMP-1 expression were inhibited after administration of chondroitin sulfate (P<0.01). Conclusions It was suggested that chondroitin sulfate might have therapeutic effects on liver fibrosis induced by alcohol in mice, by inhibiting the MDA,NO,NOS,TGF-β1,PDGF and TIMP-1 levels.