Expression And Significance Of P53 And Survivin In Junctional Nevus

Objective To study the characteristics of p53 and Survivin expression in junctional nevus and its variability in junctional nevus and malignant melanoma.To investigate the information of junctional nevus turning to malignant melanoma.Methods 47 cases of junctional nevus,13 cases of intrademal nevus and 16 cases of malignant melanoma were studyed from the First Affiliated Hospital and Provincial Hospital Department of Pathology of Anhui Medical University from 2002 to 2007.47 cases of junctional nevus and 13 cases of intrademal nevus were observed by HE staining and all junctional nevus,intrademal nevus and malignant melanoma were investigated using p53 and Survivin protein by S-P immunohistochemical technique.Results It is nested with transparence nevus cells mainly in junctional nevus histologically by HE staining.P53 and survivin protein are general expression in junctioanl nevus and malignant melanoma and did not express or express weak in intrademal nevus by S-P immunohistochemical technique.The positive reaction products are pale yellow to brown granular small localizing in the nucleus or cytoplasm.P53 protein are seen in nucleus mainly and cytoplasm little of positive cells by S-P immunohistochemical technique.Survivin protein are seen in cytoplasm mainly and nucleus little of positive cells by S-P immunohistochemical technique.The positivity rates of p53 in junctional nevus,intrademal nevus and malignant melanoma were 78.72%,30.77%,81.25%.The different expression of p53 between junctional nevus and intrademal nevus was statistically significant(χ~2=8.72,P＜0.01=,and the different expression of p53 between junctional nevus and malignant melanoma was not statistically significant(χ~2 = 0.02,P＞0.05 ).The positivity rates of Survivin in junctional nevus,intrademal nevus and malignant melanoma were 80.85%,23.08%,87.50%.The different expression of Survivin between junctional nevus and intrademal nevus was statistically significant(χ~2 = 13.15,P＜0.01 =,and the different expression of Survivin between junctional nevus and malignant melanoma was not statistically significant(χ~2=0.05,P＞0.05).P53 expression was positively correlated with Survivin expression in junctional nevus statistically(χ~2=7.81,P＜0.05,r=0.3775).Conclusion By inhibitting apoptosis in junctional nevus,mutant p53 and Survivin may be play important role and synergetic in the progress of junctional nevus and turning to malignant melanoma.It is possible to provide relevant information for malignant transformation of junctional nevus by detecting the expression of p53 and Survivin protein from the molecular level.