Treatment Of End-staged Liver Cirrhosis With Auxiliary Liver Transplantation In Rats

Treatment of end-staged liver cirrhosis with auxiliary liver transplantation in ratsPart 1 Preparation model of rats liver cirrhosisObjective:The stable and reliable model of rats terminal stage liver cirrhosis was prepared.Materials and methods:eighty male Wistar-Imamichi(WI)rats,who were six months old and four hundred to five hundred grams,were divided into two sets randomly.Sixty rats were given hypsolipoids and low protein food.The food was composed by 94.5%maizena,5.0%pork fat and 0.5% cholesterol.5%alcohol was given to take the place of water.They were eliminated blood vessels which were surrouding left renal vein and spermatic vein.Measure the portal venous pressure.After two weeks,they were given 0.35g/L Phenobarbital sodium drinks during two weeks.Then they were hypodermic injected 40%CCL₄ during four weeks,and 50% CCL₄ in the after four weeks.Dosage was adjusted by weight.The usage was twice a week during eight weeks.The first four were given by abdomen hypodermic injection and after twelve were given by four limbs hypodermic injection.Twenty rats were controls,they were given normal food and water and injected normal sodium in the same frequency,dosage and duration with another sixty rats.The weight of seventy rats were measured every week.After eight weeks,their portal venous pressure (PVP)were measured.At the same time,their hepatic tissue were obtained, then were stained by HE and Massion trichrome stain.Results:Twenty controls were all survival.Fifty-four rats were survival and another six were died.Liver cirrhosis nodus,notholobulation,portal venous pressure, and ascites formation were significantly higher than in control group. (P＜0.05) Conclusions:The stable and reliable model of rats terminal stage liver cirrhosis was prepared by association of operation,CCL₄ and drinking water.The dosage of CCL₄ was adjusted according to weight and pathological process of liver cirrhosis.Part 2 Treatment of end-staged liver cirrhosis with auxiliary liver transplantation in ratsObjective:Approach therapeutic effect of auxiliary liver transplantation to end-stage liver cirrhosis in rats and impact of splenectomy.Materials and methods:thirty six male Wistar-Imamichi(WI)rats were got randomly.Its weight were two hundred to two hundred and fifty grams.They were provided for donator liver.Eighteen rats were got randomly.Its weight were four hundred to five hundred grams.They were controls group(A).The end-staged liver cirrhosis rats from primary part were divided into three groups randomly.There were eighteen rats every group.The rats in group(B)were given granules food obtained from animal center of Kunming Medical College and were not given any measure.The rats in group(C)were treated with auxiliary liver transplantation.The rats in group(D)were treated with auxiliary liver transplantation and splenectomy.The forth week after operation,anesthetized by pentobarbital sodium,the abdominal cavity of survival rats were cut to observe appearance of donator liver and host liver.At the same time,their hepatic tissue were obtained,then were stained by HE tfichrome stain.Meanwhile, their GA,ALT,TSB,ALB,Cre,BUN,PLT,RBC and WBC were measured.The weight of survival rats were measured every week.Results:The forth week after auxiliary liver transplantation,the rats in group(A) were all survival.Five rats in group(B)were survival and survival rate of this group was 27.8%.Thirteen rats in group(C)were survival and survival rate of this group was 72.2%.Eleven rats in group(D)were survival and survival rate of this group was 61.1%. The survival rate of group(B)was significantly lower than other groups(P＜0.05).The PLT and WBC of group(D)were significantly higher than group(C).The graft liver cells accrementition, inflammation cells infiltrating,cells nucleus caryocinesis and reject reaction lightly were seen by HE trichrome stain.Hepatic lobules constitution was nomal,the graft livers became atrophic,tunica intima of vein was dropsy and thickening in Hepatic lobules, many neutrophils were seen under endomembrane and inflammation cells infiltrated surrouding Hepatic lobules.After the operation,the weight of graft livers from C,D group were significantly higher.Conclusions:End-staged liver cirrhosis can be treated efficiently by auxiliary liver transplantation in rats.By splenectomy,platelet count was increased,the rats thrombosed more easily,infection rate and death rate was increased.