The Expression Of Interleukin-2,Interleukin-10,Transforming Growth Factor-β1 And Its Receptor In Squamous Columnar Junction Epithelia Of Patients With Cervical Cancer Or Cervical Intraepithelial Neoplasia

Objectives: The expression of interleukin-2(IL-2),interleukin-lO(IL-lO) transforming growth factorβ₁(TGFβ₁) and its receptor in epithelia of cervical squamous columnar junction were investigated among the patients with cervical cancer and cervical intraepithelial neoplasia (CIN) to evaluate the local immune state of cervical lesion and the relationship of tumorigenesis. It is to be expected that the results will provide a beneficial reference to adjust clinical observation and therapeutic regimen.Methods: Fifty nine patients with cervical diseases were selected in experimental group according to TBS classification ,20 asymptomatic controls were enrolled in the study. Paired biopsy samples were taken form the cervical transformation zone. The measurement of IL-2 and IL-10 mRNA levels from the frozen tissue were done by using reverse transcription-polymerase chain reaction (RT-PCR). Western blot was used to analyse the expression of TGFβ₁ and its receptor..Results: The level of IL-2 produced by Th₁(T helper cell₁) cells were significantly lower in patients with invasive carcinoma of cervix uteri or cervical intraepithelial neoplasia than those of normal controls (P<0.05) , which were correlated with the severity of the cervical disease. The level of IL-10 produced by Th₂ (T helper cell2)cells were significantly higher in patients with invasive carcinoma of cervix uteri or cervical intraepithelial neoplasia than those of normal controls (P<0.05) , which were correlated with the severity of the cervical disease .The expression of TGFβ₁ protein in cervical carcinoma were significantly higher than those in normal cervical tissues and CIN tissues, There were no significant difference in the TGFβR II expression among CIN and controls. (P>0.05) while the expression of TGFβR II protein were significantly lower in invasive carcinoma of cervix uteri than those in CIN and controls. (P<0.01)Conclusion: The expression of IL-10 was relatively enhanced and the expression of IL-2 was inhibited in local cervical lesion of cervical invasive carcinoma and cervical intraepithelial neoplasia indicating that shifting of Th₁/Th₂ in cervical cancer. It may be one of the mechanisms of tumor escaping from immunological surveillance. In addition , the over-expression of TGFβ1 and low expression of TGFβRâ…¡may be associated with the Carcinogenesis and development of invasive carcinoma of cervix .