The Expression Of CD133 And Nestin In Human Glioma And Its Clinical Significances

Objective: To explore the expression of CD133 and nestin in human glioma cell of the patients with neurogliocytoma and to see whether or not CD133 and nestin expression associate with the differentiation and pathologic grading. It was expected that this study could reveal some essential qualities of the glioma and provide potential application for judging the malignant grades and biological treatment.Methods: The expression of CD133 and nestin was detected in 65 cases of human glioma and 19 specimens of normal brain tissues by immunohistochemistry staining.Results:â‘ The positive cell rates of the CD133 expression were 18.46% in human glioma samples and 0% in the control group (P<0.01), and nestin were respectively 23.79% and 5.16%(P<0.01).â‘¡The positive cell rates of CD133 or nestin varied accordingly to different pathological grades. There were significant difference between gradeâ… and gradeâ…¢or gradeâ…£(P<0.01). There were difference between gradeâ…¡and gradeâ…¢or gradeâ…£(P<0.01). The same significance were also found between gradeâ…¢and gradeâ…£(P<0.01). The higher pathological grades had higher positive cell rates.â‘¢There were significant difference between the positive cell rates of CD133 group and nestin group (P<0.05). The expressions of CD133 group were lower than nestin group. There was a significant correlation between CD133 group and nestin group(r=0.408,P<0.05).Conclusion: The positive correlation existed between the expression of CD133 or nestin and the pathological grading of the human glioma. Detecting CD133 and nestin in the human glioma can be used in diagnosing, judging the malignancy degree and the prognosis. CD133 and nestin will be the important cell markers of brain tumor stem cell and they will act as more substantial role in future studys.