Studies Of Proliferation Effect And Other Biology Action Changes In LoVo Cell Caused By Transforming Growth Factor α

Objective:transforming growth factor-alpha(TGF-α) is a small 50 amino acid residue long mitogenic protein,It generally exist in many human organs and body fluids.Studies confirm that TGF-αcan accommodate gastrointestinal tract physiologic function,suscitate proliferation and differentiation of gastrointestinal tract cells.It also can repair and protect the membrana mucosa of human gastrointestinal tract,and stimulate the growth and development in gastrointestinal tract of infant.In our study,we try to explore the proliferation effects of transforming growth factor-alpha from human colostrum,natural human milk,infant formula milk powder and recombinant transforming growth factor-alpha(TGF-α) on human colon epidermal cell model(LoVo) cells.And to investigate the effect of transforming growth factorα(TGF-α) on growth,total cellular RNA and protein also the ransference capability of human colon(LoVo) cells.Methods:Using MTT to assay the effects of human colostrums,natural human milk,infant formula milk powder and recombinant TGF-α(0.1,1,10 and 100ng/mL) on human colon epidermal LoVo cell proliferation in the presence or absence of TGF-αantibody.And the effects of proliferation,total cellular RNA and protein also the immigration capability of LoVo cells' were examined after the addition of TGF-αat 0.1,1(close to TGF-αmin concentration in human milk),10(close to TGF-αmax concentration inhuman milk) or 100ng/mL.Results:Whether human colostrum,natural human milk,infant formula milk powder or recombinant TGF-αcan significant stimulate the proliferation of human colon epidermal cell.And there was a dose-dependent effect of TGF-αon cell proliferation.The stimulatory effect of TGF-αwas decreased in the presence of TGF-αantibody 11±3.2%(P＜0.05),12±0.3%(P＜0.05),3±2.1% (P＞0.05) and 4±3.3%(P＞0.05).In the presence of TGF-αantibody there was a 56±13%decline in colostrum stimulated growth(P＜0.01),and a 35±3%decline in natural human milk stimulated growth(P＜0.05),and a 9±3%decline in infant formula milk powder stimulated growth(P＞0.05).After incubation with TGF-αfor 24 h,there was a significant proliferation of LoVo cells by concentration of TGF-αwere increased.The proliferation came to the top when concentration of TGF-αwere at 10ng/mL(P＜0.01).And after incubation with TGF-αfor 48 h,there was also a significant proliferation increased of LoVo cells by concentration of TGF-α.,and the proliferation came to the top when concentration of TGF-αwere at 100ng/mL(P＜0.01).And the 48h proliferation was bigger than 24h proliferation of LoVo cells.So the proliferation can increased by the time.And the total cellular RNA in the TGF-αtreatment groups was elevated from 1.67(P＜0.05) to 4.20(P＜0.01) fold of the control group as the concentration of TGF-αwere in creased.The 100ng/ml TGF-αtreatment group had significantly higher cellular protein as compared to the control and other TGF-αtreatment groups.It was 1.28 fold(P＜0.01) of the control group.And the immigration capability was notable increased by add rhTGF-αcompare with the control group.Conclusion:Neutralization of recombinant TGF-αand that present in human colostrums, natural human milk or infant formula milk powder which made mostly by bovine milk medium by TGF-αantibody led to a decrease in in vitro human colon epidermal cell proliferation.These results support that TGF-αderived from different sources has a measurable trophic effect on in vitro human gut epithelial cells.Also exogenous TGF-αcan make LoVo cells total cellular RNA and protein increased.It also can make the transference capability increased of LoVo cells...