Detection Of Different Genes And Proteins Between Pericystic Hepatocytes And Normal Liver For Human's Hydatid Disease

Objective: (l)The sheep infected the Hydatid disease under the nature condition were taken as research objects in order to observe pathologic changes in the different stages of this disease. The aim of this study was to provide the references for human's hydatid disease. (2)To construct the subtractive hybridization library of hydatid disease. (3) To screen the differentially expressed proteins between normal liver and pericystic hepatocytes and expected to find some potential proteins that play an essential role in Hepatic Hydatid Disease.Method: (1)HE staining and hydatidoscopy were used to study and analyse the results in 40 cases of the Cystic Echinococcosis and 3 cases of the normal hepatic tissues. (2)The subtractive hybridization library of hydatid disease was constructed by SSH. The differentially expressed clones were sequenced. The sequence were aligned with GenBank database using Blastn and BlastX program after removal of restriction sites.(3) In sample preparation, the fresh normal liver and pericystic hepatocyte were cut off the specimen immediately after the specimen was resected from the body and then preserved in the liquid nitrogen. These two specimen performed 2-DE after the extraction of total protein for get the result.Result and conclusion: (l)Two different ways were found according to hydatid disease's development and destination. One includes hepatic sinus dilatation, phlogocyte exudation, granuloma formation, portal area fribosis and cyst formation. The other includes hepatic sinus dilatation, phlogocyte exudation granuloma formation, hepatocyte necrosis, abscess perifocal formation. The ending of the latter would be calcify perifocal. Hepatic sinus dilatation and phlogocyte exudation are ascribed to mild lesion. Granuloma formation and portal area fribosis are ascribed to progressive lesion. Cyst formation are ascribed to severe lesion. Above is determined through the disease degree of development. (2)The subtractive hybridization library was constructed, 306 subtractive clones were gotten, 292 obverse and 14 reverse. There are 92 differential expressed clones were sequenced successfully. (3) This research compared two differentially expressed protein between normal liver and pericystic hepatocytes. To explore the establishment of protein sample preparation methods for liver tissue and optimize the experimental Parameters.