The Study Of Vascular Endothelial Growth Factor-C(VEGF-C) Protein Expression With Invasiveness And Metastasis In Human Lung Cancer

OBJECTIVE:Recent researcheres have demonstrated that multiple gene changes result in tumor genesis and progression.In the complicated courses,many genes are involved,including activation of proto-oncogenes and inactivation of antioncogenes.In clinical tumor therapy,the major factor which influences patients'prognosis is the conditions of tumor invasiveness and metastasis,and tumor biological behavior is also a major element in determination of tumor therapy plan.Many tumor experts have always been working hard at the early diagnosis of tumor invasiveness and metastasis,In order to improve tumor prognosis and prolong patients'life as much as possible.Therefore,tumor markers which can provide information on the conditions of tumor invasiveness and metastasis have always been emphasized by many tumor experts and become a hot researching subject,among which isVEGF-C.Vascular endothelial growth factor-C (VEGF-C),cloned by Kaipainen in 1995,was found as a unique and specific lymphatic vessel growth factor so far.In recent years,the research also shows that high expression of VEGF-C in the solid tumor cell closely relate to the proliferation and metastasis of the tumor cells.Lung cancer is the most usually malignant tumor.Whether the expressions of VEGF-C protein relates to the invasive growth and lymph node metastasis of the lung cancer are not reported.The research tested expressions ofVEGF-C gene of 65 cases in lung cancer tissues,adjacent tissues of cancer and normal lung tissues by immunohistochemical staining.METHODS:The expression of VEGF-C protein in 65 cases of lung cancer,adjacent tissues of cancer and normal tissues was tested by immunohistochemical SABC method and analysed by clinico-pathological characteristics and prognosis of lung cancer.RESULTS:The results of immunohistochemical SABC method are that the rate of expression of VEGF-C protein in tumor tissues(56.9%)was significantly higher than that in adjacent tissues of cancer(12.3%)and normal tissues(6.2%)(P＜0.05);It was shown that VEGF-C protein expression did significantly not correlate with the type(54.8%,56.0%,66.7%,66.7%)(P＞0.05)and grade of the tumor(58.8%,56.5%,56.3%,55.6%)(P＞0.05);but it correlated with the clinical stage(37.2%)(P＜0.05)and survival time of the patients(36.1%)(P＜0.05).It was shown that VEGF-C protein expression did significantly not correlate with the type(61.3%,60.0%,66.7%,66.7%)(P＞0.05)and grade ofthe tumor(58.8%,60.9%,62.5%,66.7%)(P＞0.05);but it correlated with the clinical stage(41.9%)(P＜0.05)and survival time of the patients(66.7%)(P＜0.05)CONCLUSIONS:The results suggested that there be over expression of VEGF-C protein in lung cancer tissues,and which perhaps participate in regulation of tumorigenesis,progression in lung cancer The expression of VEGF-C protein be used as an useful marker of the biological behavior of lung cancer and as an independent prognosis factor for the patients with lung cancer...