Effects Of Remifentanil Preconditioning On The Content Of H-FABP In Serum And NF-κB,HSP70 In The Cardiac Muscle Tissue Of Canines With Myocardial Ischemia Reperfusion Injury

Objective To observe the effects of remifentanil preconditioning on the content of H-FABP in serum and NF-κB,HSP70 in the cardiac muscle tissue of canines with acute myocardial ischemia reperfusion injury(MIRI).To investigate the mechanism of action about remifentanil's anti-MIRI.Methods 24 healthy hybrid canines were randomly divided into four groups(n=6):sham operated group(S),ischemia-reperfusion group(I/R),remifentanil preconditioning groupⅠ(RPC_Ⅰ) and remifentanil preconditioning groupⅡ(RPC_Ⅱ).The model of MIRI was established by occluding the anterior descending branch of left coronary artery with 30min ischemia and 2h reperfusion.In RPC_Ⅰgroup,30min's infusion of remifentanil of 1μg·kg^-1·min^-1 was administrated before ischemia while that was 2μg·kg^-1·min^-1in RPC_Ⅱgroup.The contents of H-FABP in serum were measured at four time points:Pre-preconditioning,30 min after ischemia,1 h and 2 h after referfusion respectively by enzyme linked immunosorbent assay(ELISA).The expression of NF-κB and HSP70 in the cardiac muscle tissue were measured at 2 h after referfusion by immunohistochemistry.Results Compared with the sham operated group,the contents of H-FABP,NF-κB and HSP70 of the other three groups all heightened significantly(P＜0.05).Compared with the schemia-reperfusion group,remifentanil preconditioning groupⅠand remifentanil preconditioning groupⅡ'levels of H-FABP reduced(P＜0.05),and the expression of NF-κB attenuated(P＜0.05),but the expression of HSP70 strengthened(P＜0.05).Compared with the remifentanil preconditioning groupⅠ,remifentanil preconditioning groupⅡ'contents of H-FABP reduced(P＜0.05),but the expression of NF-κB demonstrated to be no significant variation(P＞0.05),however the expression of HSP70 strengthened(P＜0.05).Conclusion Remifentanil preconditioning can significantly decrease the levels of H-FABP in serum of canines with acute myocardial ischemia reperfusion injury,can attenuate the activity of NF-κB in the cardiac muscle tissue in the early phase of reperfusion,and can strengthen the expression of HSP70 in the cardiac muscle tissue in the early phase of reperfusion.