The Expression Of HGF/c-Met In Gastric Carcinoma And The Correlation With Lymphangiogenesis And Lymph Node Metastasis

Objective:To detect the expressive levels of hepatocyte growth factor and c-Met and D2-40 in gastric carcinoma;To detect the MLD and its correlation with clinicopathological factors in gastric carcinoma;To investigate the possible mechanism of lymph node metastasis in gastric carcinoma.Methods:Immunohistochemistry method was used to detect the expression of HGF and c-Met and D2-40 in gastric cancer tissue and the normal tissues near the tumor borderline,with microlymphatic density (MLD)counted.Results(1).HGF and c-Met was expressed mainly in cytoplasm of gastric carcinoma cells.The expressive rate of HGF and c-Met in gastric cancer were 67.3%and 78.8%,respectively.(2).The expression of HGF had the positive correlation with histologic types,infiltration depth,clinical stage and lymph node metastasis(P＜0.05).No correlation was found in age,tumor diameter(P＞0.05).(3).The expression of c-Met had the positive correlation with histologic types and lymph node metastasis(p＜0.05).No correlation was found in age,tumor diameter,infiltration depth and clinical stage(P＞0.05).(4).The MLD in the gastric cancer was higher than that in the normal tissues near the tumor borderline(P＜0.05).The MLD had no correlation with infiltration depth and clinical stage(P＞0.05).The positive correlation was found in histologic types and lymph node metastasis(p＜0.05)(5).In 52 cases of gastric cancer,there were 31 cases where the expression of HGF and c-Met was positive simultaneously.The simultaneously negative was 7 cases.Correlation coefficient showed that HGF had a positive correlation with c-Met in gastric cancer(r=0.342, P＜0.05).(6).In gastric cancer,The MLD in the HGF-positive group was higher than that in the HGF-negative group(P＜0.05)Conclusion(1).HGF and c-Met are expressed in plasma in most gastric cancer cell.(2).The expression of HGF has correlation with histologic types, infiltration depth,clinical stage and lymph node metastasis in the gastric carcinoma.The expression of c-Met has correlation with histologic types and lymph node metastasis in the gastric carcinoma.(3).The MLD in the gastric cancer was higher than that in the normal tissues near the tumor borderline.The MLD has the positive correlation with histologic types and lymph node metastasis.(4).The MLD in the gastric cancer has correlation with the expression of HGF,but it has no correlation with the expression of c-Met.