| Objective:The gastric carcinoma is one of the most common malignant tumors in our country. The rate of incidence and death is the highest in all kinds of the alimentary tract malignant tumors. At present, the happening and development of gastric cancer is still emphasis of study. The happening and development of gastric cancer is a progress in whichmany factors and processes and various mechanisms were involved. KAI1/CD82 could combine with many integrin members and epithelium growth factor receptors to form complex which mediate cell adherence via PKC, IP4 signaling, so it could prevent the detachment of tumor cells from their primary focus; it could also inhibit tumor cells migration and proliferation in the secondary focus, and thus plays important roles in inhibiting the tumor metastasis and invasion. The abnormal expression of N-Cadhein can promote the tumor invasion and metastasis and turn tumor cells into invasive malignant phenotype.This kind of action is contrary to the E-Cadherin and it is more obvious and direct than E-Cadherin low-level expression.Now there were little reports about the Correlations between the expressions of KAI1/CD82 and N-Cadherin in gastric carcinoma and the prognosis. Therefore, in this study we detect the expressions of KAI1/CD82 and N-Cadherin in gastric carcinoma tissues and normal gastric mucosa by S-P immunohistochemical technique analyze the Correlations between the expressions of tumor cells, the Correlations between the expressions and the biological behavior of gastric carcinoma and the Correlations between the expressions and the prognosis. We studied important theoretical and clinical meanings in the happening, invasion and metastasis of gastric cancerMethods:In this study, we select 50 samples of gastric carcinoma, 50 samples of normal gastric mucosa(>5.0cm). All patients had gastrectomy in the 4th hospital of HeBei Medical University from October 2006 to August 2007. All these samples are without chemotherapy or radiotherapy before surgery, which are proved adenocarcinoma by pathology. In this study, we use S-P immunohistochemical technique to detect the expressions of protein KAI1/CD82 and N-Cadherin in gastric carcinoma tissues, normal gastric mucosa. The Correlations between the expressions of KAI1/CD82 and N-Cadherin and the biological behavior of gastric carcinoma were analyzed, including patients'sex, age, tumor location, size, gross type, differentiation degree, the depth of invasion and TNM stage etc. We still analyzed the Correlations between the expressions of KAI1/CD82 and N-Cadherin. All statistical analyses were carried out with SPSS 13.0 and P<0.05 were considered significant. Results:1 The positive rates of KAI1/CD82 were 54% in tissues of gastric carcinoma, which were significantly lower than the rates of 96% in normal gastric mucosa (p<0.05).2 The positive rates of N-Cadherin were 68% in tissues of gastric carcinoma, which were significantly higher than the rates of 26% in normal gastric mucosa (p<0.05).3 In gastric carcinoma tissues, 36% of KAI1/CD82 in gastric carcinoma tissue existed in the tumor bigger than 5.0cm, and the percentage was lower than 72% in the tumor smaller than 5.0cm; the comparison of the two shows significant difference (P<0.05). The expressions of KAI1/CD82 in the group with infiltrating type (38.46%) were significantly lower than localization type (70.83%, P<0.05); the expressions of KAI1/CD82 in the group with poor differentiation (41.38%) were significantly lower than well differentiation (71.43%, P<0.05); the expressions of KAI1/CD82 in the group of T3+T4 (46.34%) were significantly lower than T1+T2 (88.89%, P<0.01); the expressions of KAI1/CD82 in the group with lymph node metastasis (45%) were significantly lower than without lymph node metastasis (90%, P<0.05); the expressions of KAI1/CD82 in the group of III-IV stage (46.34%) were significantly lower than group of I-II stage (88.89%, P<0.05).4 In gastric carcinoma tissues, 84% of N-Cadherin in gastric carcinoma tissue existed in the tumor bigger than 5.0cm, and the percentage was higher than 52% in the tumor smaller than 5.0cm. The comparison of the two shows significant difference (P<0.05) ; the expressions of N-Cadherin in the group with infiltrating type (80.77%) were significantly higher than localization type (54.17%, P<0.05) ; the expressions of N-Cadherin in the group with poor differentiation (82.76%) were significantly higher than well differentiation (47.62%, P<0.05); the expressions of N-Cadherin in the group of T3+T4 (75.61%) were significantly higher than T1+T2 (33.33%, P<0.05); the expressions of N-Cadherin in the group with lymph node metastasis (75.5%) were significantly higher than without lymph node metastasis (30%, P<0.05); the expressions of N-Cadherin in the group of III-IV stage (78.05%) were significantly higher than group of I-II stage (22.22%, P<0.05).5 No correlations were found between expression levels of KAI1/CD82 and N-Cadherin in gastric carcinoma tissues and patients'sexes, ages and tumor locations. (P>0.05).6 A significant negative correlations were observed between the expression of KAI1/CD82 and N-Cadherin protein in gastric carcinoma tissues (rs= -0.493, P<0.01).Conclusion:1 The expressions of KAI1/CD82 in gastric carcinoma tissues were lower than those in normal gastric mucosa. It shows the inactivation of KAI1/CD82 participate the happening and the development of gastric cancer. The expressions of N-Cadherin in gastric carcinoma tissues were significantly higher than those in normal gastric mucosa. The abnormal expression of N-Cadhein can promote the tumor invasion and metastasis and turn tumor cells into invasive malignant phenotype.2 The expressions of KAI1/CD82 in gastric carcinoma tissues become significantly lower along with differentiation degree to lower, the depth of invasion to deeper, lymph node metastasis. The expressions of N-Cadherin in gastric carcinoma tissues become significantly higher along with differentiation degree to lower, the depth of invasion to deeper, lymph node metastasis,. It indicates the ability of the invasion and metastasis of gastric carcinoma become higher, which the low expression of KAI1/CD82 and the high expression of N-Cadherin.3 The expressions of KAI1/CD82 in gastric carcinoma tissues become significantly lower along with TNM stage to higher. The expressions of N-Cadherin in gastric carcinoma tissues become significantly higher along with TNM stage to higher. It indicates TNM stage of gastric carcinoma become higher, which the low expression of KAI1/CD82 and the high expression of N-Cadherin.4 No correlations were found between expression levels of KAI1/CD82, N-Cadherin in gastric carcinoma tissues and patients'sexes, ages and tumor locations. (P>0.05).5 In gastric carcinoma tissues, the expressions of KAI1/CD82 and N-Cadherin have negative correlation. It shows the expressions of KAI1/CD82 and N-Cadherin in gastric carcinoma have the antagonism in invasion and metastasis of gastric carcinoma.In conclusion, the abnormal expressions of KAI1/CD82 and N-Cadherin in gastric carcinoma tissues play important roles in invade and metastasis of gastric carcinoma and the prognosis of gastric carcinoma. Unite examining the expressions of KAI1/CD82 and N-Cadherin in gastric carcinoma tissues can be useful in prognosticating the invasion and metastasis of gastric carcinoma, evaluating the prognosis of gastric carcinoma and guiding the reasonable comprehensive treatment. |
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