Expression And Clinical Significance Of KAI1/CD82 And FAK In Hepatocellular Carcinoma

Objective: Primary hepatic carcinoma (PHC) is the most common malignant tumor in China, morbidity and mortality of hepatoma have elevated tendency year by year in recent years. In China, 130 thousand patients dead of PHC per year, and the mortality is occupies 18.8% and in the second place of malignant tumors. Hepatocellular carcinoma (HCC) occupies 91.5 % of Primary hepatic carcinoma in our country. At present, there are no efficacious therapies to cure HCC because of the bionomics of powerful infiltration, easy recrudescence, metastasis and so on. Exacts molecular mechanisms of carcinogenesis, development, invasion and metastasis are not clarified. It has become the oncological hot topic to research the etiopathogenisis, pathogenesis, explore and original therapeutics of HCC. Oncogenesis and metastasis are multigenic and multifactorial results. KAI1/CD82 gene as an anti-metastasis gene belongs to transmembrane 4 superfamily (TM4SF), appears inhibit metastatic by adjust adhere between cells and restrain tumor cells which divorced from the original position. Focal Adhesion Kinase (FAK) is a nonreceptor tyrosine kinase. As a central molecule of integrin mediated signal transduction, FAK is a protein kinase correlated to cell canceration, cell differentiation, infiltration and metastasis. Expression and clinical significance of KAI1/CD82 and FAK in human HCC had not been reported in literature. The experimental aims are investigate the expressed dependablity of KAI1/CD82 and FAK, research the dependablity between clinical characteristics and pathological characteristics as well as the role of KAI1/CD82 and FAK in occurrence and development of HCC.Methods: Sixty-four HCC tissue samples and thirty-eight para-cancerous tissue samples were obtained from primary HCC patients undergoing surgical resection. Twenty normal hepatic tissue samples were taken from patients with hemangiomas of liver. S-P method of Immunohistochemistry was used to detect expression of KAI1/CD82 and FAK. Statistical analysis was used to determine the relation between their expression and clinicopathological characteristics.Results: 1 Expression of KAI1/CD82 protein and its correlation to clinicopathological characteristicsKAI1/CD82-positive stain was observed in twenty-one of 64 (32.8%) HCC tissue samples, was in thirty-three of 38 (86.8%) in para-cancerous tissue samples and was in eighteen of 20 in normal hepatic tissue. Expressed rate of KAI1/CD82 decreased significantly in HCC tissue, compared with that in para-cancerous tissue and normal hepatic tissue (P<0.05, P<0.05), but it had no significant difference between para-cancerous tissue and normal hepatic tissue (P>0.05).Expressed rate of KAI1/CD82 protein in HCC had no correlation to sex, age, HBsAg, AFP or size of tumor; it had correlation to pathological degree, vein embolus, intrahepatic and lymphatic metastasis (P<0.05).2 Expression of FAK protein and its correlation to clinicopathological characteristicsFAK-positive stain was observed in forty-eight of 64 (75%) in HCC tissue samples, was in fifteen of 38 (39.5%) in para-cancerous tissue samples and was in three of 20 (15%) in normal hepatic tissue. Expression of FAK increased signifi- cantly in HCC tissue, compared with that in para-cancerous tissue and normal hepatic tissue (P<0.05, P<0.05), but it had no significant difference between para-cancerous tissue and normal hepatic tissue (P>0.05).Expression rate of FAK protein in HCC had no correlation to sex, age, HBsAg, AFP or size of tumor it had correlation to pathological degree, vein embolus, intrahepatic and lymphatic metastasis (P<0.05).3 Correlation between expression of KAI1/CD82 and FAK protein in HCC tissueA significant negative correlation was observed between expression of KAI1/CD82 and FAK in HCC (rs=-0.357, P=0.004).Conclusion: 1 Expression of KAI1/CD82 was decreased significantly in HCC tissue, compared with that in para-cancer- ous tissue and normal hepatic tissue. Expression of KAI1/CD82 protein in HCC tissue was correlated to pathological degree, vein embolus, intrahepatic and lymphatic metastasis.The abnormal expression of KAI1/CD82 may be involved in infiltration and metastasis of HCC.2 Expression of FAK increased significantly in HCC tissue, compared with that in para-cancerous tissue and normal hepatic tissue. Expression of FAK in HCC tissue was correlated to pathological degree, vein embolus, intrahepatic and lymphatic metastasis. The abnormal expression of FAK may be involved in the oncogenesis, development, infiltration and metastasis of HCC.3 KAI1/CD82 and FAK expression are negative correlation in malignant development of HCC, both of them utend in common at infiltration and metastasis of HCC.