Fingerprints Of Compound Hawthorn Preparation By HPLC-DAD-MS/MS

The HPLC-DAD-MS fingerprint of compound hawthorn preparation was established with chromatographic column Aligent XDB 4.6mm×250mm,5μm, C18. The flow rate is 1.0ml/min and the detection wavelength is 260nm.The gradient elution mode was applied in chromatographic separation, the mobile phase was0.1% formic acid acetonitrile(B)- 0.1% formic acid water (A) (v/v): 0min,5%:95%;10min,10%:90%;90min,17%:83%;100min,19%:81%;110min,19%:81%;130min,23%:77%;140min,23%:77%;160min,30%:70%.180min,40%:60%;200min,100%:0%;200min时100%:0%.The optimized MS parameters mode were as follows: ion spray voltage, 4.5 kV; sheath gas, 30 arbitrary units; auxiliary gas, 5 arbitrary units; capillary temperature, 300℃,Capillary voltage, 35V in the whole analytical times. For full scan MS analysis, the spectra were recorded in the range of m/z 150–1200. The isolation width of precursor ions was 3.0 units. MSn data were acquired in the automatic data-dependant mode. The method is accurate and reproducible,providing a new reference for quality control and further research of Compound Hawthorn Preparation。36 compounds were identified with the help of HPLC-DAD-MS fingerprint,and the herb which each chemical come from is confirmed.