Study On The Expression Of PCNA,p53 In The Helicobacter Pylori Raised Erosive Gastritis With Spleen-Stomach Dampness-Heat Syndrome

Objiective:To study the relationship between the expression of PCNA, p53 in the H. pylori Raised Erosive Gastritis with spleen-stomach dampness heat syndrome. To discuss the difference between spleen-stomach dampness-heat(SSDH)group and spleen stomach qi-deficiency(SSQD)group of pathological and molecular biology,search for objective indicators of TCM type, with the hopeless of help preventting and treating in the Helicobacter Pylori Raised Erosive Gastritis.Method:80 H.pylori Raised Erosive Gastritis patients were selected as research subjects. According to the differentiation standards of Raised Erosive Gastritis of TCM, 44 cases coincided to spleen-stomach dampness-heat(SSDH)group,36 cases coincided to spleen stomach qi-deficiency(SSQD)group,and 18 healthy persons as control group. Antral mucosal biopsies were obtained during endoscopy in these patients. Rapidurease tests and histological tests were used for detection of the Hp. The specimens of gastric antral mucosa were obtained for pathological diagnosis and immunohistochemical technique(PCNA, p53).The correlation among above index signs were analyzed.Results:1. The degree of atrophy in the SSDH group (34.1%)were more serious than in the SSQD group(13.9%) (p<0.05),The percentage of intestinal metaplasia in the SSDH group (36.4%)werc more serious than that in the SSQD group(13.9%),too(p<0.05).2. The expression of PCNA in Raised Erosive Gastritis with SSDH was enhanced and obviously different with in the SSQD group and in the control group, it was also obviously different between in the SSQD group and in the control group.3. The expression of p53 in the control group was negative or weak, while that in Raised Erosive Gastritis patients with SSDH and SSQD was enhanced and was obviously different with in the control group(/7<0.01),between in the SSDH group and in the SSQD group was also indifferent(p<0.05),and the SSDH group is higher than the SSQD group.Conclusion:1. During the development in the SSDH was much faster and serious. The rease may be a close coordination between SSDH and inflammation.2. PCNA and p53 are maybe important molecular events during the development in the SSDH of H.pylori Raised Erosive Gastritis.both can be served as monitoring indicatos in prognosis and treatment.3. Gastric cell loss of balance with proliferation and apoptosis in the SSDH,maybe resulting higher danger of carcinogenesis.