Study On The Expression Of IL-8, TNF-α, And IL-10 In The Raised Erosive Gastritis With Spleen-Stomach Dampness-Heat Syndrome

Objective:To study the relationship between the expression of IL-8,TNF-a, IL-10 and the Raised Erosive Gastritis (REG)with spleen-stomach dampness heat syndrome.Method:70 Raised Erosive Gastritis patients whoses syndromes meet with spleen stomach-dampness-heat(SSDH) and spleen deficiency(SD) accroding to TCM dialectics were selected as research subjects.There were 34 patients in spleen-stomach dampness-heat(SSDH) group,36 patients in spleen deficiency(SD) group. And 13 healthy patients were considered as the control group. All subjects accepted gastroscopy examination.We got the samples from the gastric antrum,during the gastroscopy examination;and then the samples were used to do rapid urease test, detect the existence of Hp with histological stain method,do pathologic diagnosis and detect the expression of IL-8,TNF-a and IL-10 with immunohistochemistry.Results:1 There were no difference between the Raised Erosive Gastritis group and the control group about the expression of IL-8 (p>0.05). There were no significantly difference among the Hp positive group, the Hp negative group and the control group about the expression of IL-8(p>0.05). The expression of IL-8 in the SSDH group and the SD group were both higher than the control group, but the difference was not significant (p>0.05). The expression of IL-8 in the SSDH group and the SD group have no difference(p>0.05. In the SSDH, the expression of IL-8 between the Hp negative group and the control group was not different(p>0.05),and the result was the same in the SD group.2 The expression level of TNF-a in the Raised Erosive Gastritis group was obviously higher than the control group(p<0.01),the expression level of TNF-a in the Hp positive group and the Hp negative group were both higher compared with the control group,while the Hp negative group is higher than the Hp positive group (p<0.05). Both the SSDH group and the SD group were obviously different from the control group about the expression of TNF-a (p<0.01).Though the SSDH group expressed more TNF-a than the SD group, the different was not significan(p>0.05). In the SSDH group, the expression of TNF-a in the Hp negative group was enhanced and obviously different from the Hp positive group (p<0.01).In the SD group, the expression of TNF-a between the Hp negative group and the Hp positive group was not different (p<0.01).3 The expression of IL-10 in the Raised Erosive Gastritis group was obviously higher than the control group(p<0.01).The expression of IL-10 in the Hp positive group and the Hp negative group were both higher compared with the control group,and the Hp negative group is higher than the Hp positive group (p<0.05).The expression of IL-10 in the SSDH group and the SD group was both enhanced and obviously different from the control group(p<0.01).The SSDH group expressed more IL-10 than the SD group, but the different was not significant(p>0.05). There were no difference between the SSDH group and the SD group about the expression of IL-10 (p>0.05).In the SSDH group, The expression of IL-10 was not different between the Hp positive group and the Hp negative group (p>0.05).In the SD group, the expression of IL-10 in the Hp negative group was enhanced and obviously different fome the Hp positive group(p<0.05).Conclusion:1 IL-8 may not be a major proinflammatory cytokine in the the occurrence and development of REG2 TNF-αmay play an important role in promoting the occurrence and development of REG3 IL-10 may an important role in preventing tissue from damage in the REG4 It was not different between the REG SSDH group and the REG SD group about the expression of IL-8,TNF-a and IL-10.